HEL46.11

General#

Cell Line

hPSCreg Name ULBi003-A
Alternative name(s)
HEL46.11
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 18th February 2019
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Provider

Generator Université Libre de Bruxelles (ULB)

External Databases

BioSamples SAMEA5573418
Cellosaurus CVCL_UL37

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male
Ethnicity White

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

External Databases (Donor)

BioSamples SAMEA5573419

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell line name CCD-1112Sk
Other names
Derived from same source line (potentially other lot and donor, see below):
Source cell type
dermal fibroblast
Dermal fibroblasts are the major cell type in dermis and are commonly accepted as terminally differentiated cells.
Source cell origin
prepuce of penis
A retractable double-layered fold of skin and mucous membrane that covers the glans penis and protects the urinary meatus when the penis is not erect.
Synonyms
  • penile prepuce
  • prepuce of male
  • preputium penis
Source cell line vendor ATCC

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-1
Yes
TRA 1-60
Yes
Self-renewal
Unkown
Endoderm
Unkown
Mesoderm
Unkown
Ectoderm score
Positive
Data analysis report
Pluripotency markers endo,meso,ectoderm.png
Self-renewal (Mitosis with DAPI), Endoderm (SOX17), Mesoderm (Vimentin), Ectoderm (Tubulin)
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
Protocol or reference
SOX17 (Alexa568 + DAPI).tif
SOX17 (Alexa568 + DAPI)
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Vimentin
Yes
Protocol or reference
Vimentin (Alexa568 + DAPI).tif
Vimentin (Alexa568 + DAPI)
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Tubulin
Yes
Protocol or reference
Tubulin (Alexa488 + DAPI).tif
Tubulin (Alexa488 + DAPI)

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY normal

Other Genotyping (Cell Line)