MIFF1, ShiPS-MIFF1, MIFF-1

General#

Cell Line

hPSCreg Name UOSi001-A
Alternative name(s)
MIFF1, ShiPS-MIFF1, MIFF-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 16th January 2018
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Provider

Generator University of Sheffield (UOS)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_1E69

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions:

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian, White

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA103885930

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell line name CCD-1112Sk
Other names
Derived from same source line (potentially other lot and donor, see below):
Source cell type
foreskin fibroblast
Fibroblast from foreskin.
Synonyms
  • BJ fibroblast
Source cell origin
prepuce of penis
A retractable double-layered fold of skin and mucous membrane that covers the glans penis and protects the urinary meatus when the penis is not erect.
Synonyms
  • penile prepuce
  • prepuce of male
  • preputium penis
Age of donor (at collection) neonate
Source cell line vendor ATCC

Reprogramming method

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA
mRNA

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells mouse embryonic fibroblasts
Cellfinder Ont Id: CF_43885
Passage method Enzymatically
Collagenase
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)