UHi001-A, HEL24.3-SOX2-nTdT-C9-H5, HEL24.3-SOX2-nTdT

General

Cell Line

hPSCreg name UHi006-A-1
Cite as:
UHi006-A-1 (RRID:CVCL_LJ89)
Alternative name(s)
UHi001-A, HEL24.3-SOX2-nTdT-C9-H5, HEL24.3-SOX2-nTdT
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UHi006-A
(HEL24.3)
UCSFi001-A-37
(AICS-0074-026)
UHi006-A-2
(HEL24.3-OCT4-nEmGFP-A-G3, UHi001-B, HEL24.3-OCT4-nEmGFP)
RUESe002-A-6
(RUES2-GLR)
BIHi001-A
(BCRT-3, BCRT#1)
UOSi001-B
(MIFF3, MIFF-3, ShiPS-MIFF3)
UOSi001-A
(MIFF1, ShiPS-MIFF1, MIFF-1)
BIHi001-A-1
(iBCRT Cas9v1-3G-Kl.16)
Last update 12th August 2020
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Provider

Generator University of Helsinki (UH)
Owner University of Helsinki (UH)
Distributors
Derivation country Finland

External Databases

BioSamples SAMEA104134270
Cellosaurus CVCL_LJ89
Wikidata Q54990146

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian, White

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA103885930

Ethics

Also have a look at the ethics information for the parental line UHi006-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line UHi006-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Notes on reprogramming vector detection http://www.sciencedirect.com/science/article/pii/S1873506115000719

Vector free reprogramming

Other

Selection criteria for clones http://www.sciencedirect.com/science/article/pii/S1873506115000719
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
Differentiation Potency
Endoderm
Ont Id: NCIT_C12706
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Mesoderm
Ont Id: NCIT_C12750
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Ectoderm
Ont Id: NCIT_C12703
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
SOX2 (target)
Gene knock-in
T2A-NLS-tdTomato-F2A-Pac knocked in at SOX2 stop codon
CRISPR-associated (CRISPR/Cas) System