Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9
Summary
SOX2 is an important transcription factor involved in pluripotency maintenance, pluripotent reprogramming and differentiation towards neural lineages. Here we engineered the previously described HEL24.3 hiPSC to generate a SOX2 reporter by knocking-in a T2A fused nuclear tdTomato reporter cassette before the STOP codon of the SOX2 gene coding sequence. CRISPR/SaCas9-mediated stimulation of homologous recombination was utilized to facilitate faithful targeted insertion. This line accurately reports the expression of endogenous SOX2 and therefore constitutes a useful tool to study the SOX2 expression dynamics upon hiPSC culture, differentiation and somatic cell reprogramming. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Balboa D, Weltner J, Novik Y, Eurola S, Wartiovaara K, Otonkoski T |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2017 Jul;22:16-19 |
| PubMed | 28952927 |
| DOI | 10.1016/j.scr.2017.05.005 |