RUES2-GLR
RUESe002-A-6
General
Cell Line |
|
| hPSCreg name | RUESe002-A-6 |
| Cite as: | RUESe002-A-6 |
| Alternative name(s) |
RUES2-GLR
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
RUESe002-A (RUES2) UCSFi001-A-37 (AICS-0074-026) UHi006-A-1 (UHi001-A, HEL24.3-SOX2-nTdT-C9-H5, HEL24.3-SOX2-nTdT) RUESe002-A-4 (RUES2-mCitrine-SMAD2; H2B-mCherry) RUESe002-A-5 (RUES2-RFP-SMAD1-H2B-mCitrine) |
| Last update | 13th March 2023 |
| User feedback | |
Provider |
|
| Generator | The Rockefeller University (RUES) |
| Owner | The Rockefeller University (RUES) |
| Distributors | |
| Derivation country | United States |
External Databases |
|
| BioSamples | SAMEA112765832 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
For use in teaching and non-commercial use only; available for diagnostic or therapeutic use only with prior permission by the Provider. |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Disease associated phenotypes | no phenotypes |
| Family history | no |
| Is the medical history available upon request? | no |
| Is clinical information available? | no |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Yes
46Xx
Karyotyping method:
G-Banding
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
|
External Databases (Donor) |
|
| BioSamples | SAMEA104387770 |
Ethics
Also have a look at the ethics information for the parental line
RUESe002-A
.
| Is there an MTA available for the cell line? | No |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution | For use in teaching and non-commercial use only; available for diagnostic or therapeutic use only with prior permission by the Provider. |
hESC Derivation
|
The source cell information can be found in the parental cell line
RUESe002-A.
|
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
CF1 PMEF |
| Passage method | Mechanically |
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
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| NANOG |
Yes |
|
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| POU5F1 (OCT-4) |
Yes |
|
|
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 18
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
|
Genetic Modification
| Genetic modifications not related to a disease |
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