RUES2-mCitrine-SMAD2; H2B-mCherry


Cell Line

hPSCreg name RUESe002-A-4
Cite as:
Alternative name(s)
RUES2-mCitrine-SMAD2; H2B-mCherry
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 22nd November 2021
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Generator The Rockefeller University (RUES)

External Databases

BioSamples SAMEA10794674
Cellosaurus CVCL_C1W4
Wikidata Q114312931

General Information

* Is the cell line readily obtainable for third parties?
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history no
Is the medical history available upon request? no
Is clinical information available? no

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?

External Databases (Donor)

BioSamples SAMEA104387770


Also have a look at the ethics information for the parental line RUESe002-A .
Is there an MTA available for the cell line? Yes
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line RUESe002-A.

Culture Conditions


No characterisation data could be found for this subclone. Please open parental cell line RUESe002-A .


Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
SMAD2 (target)
Gene knock-in
Chromosome 18: 47,808,957-47,931,146 reverse strand.
mCitrine-SMAD2 reporter line, CRISPR/Cas9 was used to fuse a cassette containing a puromycin resistance gene (PuroR), a T2A self-cleaving peptide, and an mCitrine fluorescent protein onto the N-terminus of SMAD2, so that the locus produces both an mCitrine-SMAD2 fusion protein together with PuroR.
CRISPR-associated (CRISPR/Cas) System