RUES2-RFP-SMAD1-H2B-mCitrine

General#

Cell Line

hPSCreg Name
RUESe002-A-5
Alternative name(s)
RUES2-RFP-SMAD1-H2B-mCitrine
Cell line type Human embryonic stem cell (hESC)
Last update 22nd November 2021
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Provider

Generator The Rockefeller University (RUES)

External Databases

BioSamples SAMEA10794729
CLO CLO_0103594

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history no
Is the medical history available upon request? no
Is clinical information available? no

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

External Databases (Donor)

BioSamples SAMEA104387770

Ethics#

Also have a look at the ethics information for the parental line RUESe002-A .
Is there an MTA available for the cell line? Yes

hESC Derivation#

The source cell information can be found in the parental cell line RUESe002-A.

Culture Conditions#

Characterisation#

No characterisation data could be found for this subclone. Please open parental cell line RUESe002-A

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
SMAD1 (target)
Gene knock-in
Chromosome 4
Chromosome 4: 145,481,194-145,559,176 forward strand.
CRISPR/Cas9 mediated genome engineering was used to fuse a cassette containing a blasticidin resistance gene (BsdR), a T2A self-cleaving peptide, and a tagRFP fluorescent protein onto the N-terminus of SMAD1, so that the locus produces both a tagRFP-SMAD1 fusion protein together with BsdR.
CRISPR-associated (CRISPR/Cas) System