RUES2

General#

Cell Line

hPSCreg Name RUESe002-A
Alternative name(s)
RUES2
Cell line type Human embryonic stem cell (hESC)
Last update 16th January 2018
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Provider

Generator The Rockefeller University (RUES)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA104387770
Cellosaurus CVCL_B810
NIHhESC NIHhESC-09-0013

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions: Available for clinical and commercial research
Subclones

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history no
Is the medical history available upon request? no
Is clinical information available? no

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46XY
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

External Databases (Donor)

BioSamples SAMEA104387770

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2005-08-30
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Expansion status 3
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology a
Stephenson 2007
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM
Derived under xeno-free conditions?
No
Derivation under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Feeders, matrigel/geltrex, laminin521
Feeder cells CF1 PMEF
Passage method Mechanically
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
Score:
Marker Present Absent
mCpG
OCT4
derivation and in vitro and in vivo characterization
Method documentation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro directed differentiation
Neuroepithelial Stem Cell
Ont Id: CL_0002259
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Neuron
Ont Id: CL_0000540
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Retinal Pigment Epithelium
Ont Id: BTO_0001177
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes