RUES2
RUESe002-A
General#
Cell Line |
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| hPSCreg Name | RUESe002-A |
| Alternative name(s) |
RUES2
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| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 16th January 2018 |
| User feedback | |
Provider |
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| Generator |
The Rockefeller University (RUES)
Contact:
The Rockefeller University (RUES) |
| Distributors | |
| Derivation country | United States |
External Databases |
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| BioSamples | SAMEA104387770 |
| Cellosaurus | CVCL_B810 |
| NIHhESC | NIHhESC-09-0013 |
General Information |
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| Publications |
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| Projects | |
| Is the cell line available in principle? | Available with restrictions: Available for clinical and commercial research |
| Subclones | |
Donor Information#
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Disease associated phenotypes | no phenotypes |
| Family history | no |
| Is the medical history available upon request? | no |
| Is clinical information available? | no |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
46XY
Karyotyping method:
G-Banding
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
|
External Databases (Donor) |
|
| BioSamples | SAMEA104387770 |
Ethics#
We are currently revising this section.hESC Derivation#
| Date of derivation | 2005-08-30 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
No |
| Expansion status |
3
|
| ICM morphology |
A
|
| Trophectoderm morphology |
a
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| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
| Derived under xeno-free conditions? |
No |
| Derivation under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
| Surface coating | Feeders, matrigel/geltrex, laminin521 |
| Feeder cells |
CF1 PMEF |
| Passage method | Mechanically |
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
derivation and in vitro and in vivo characterization
Method documentation
Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
|
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