HEL24.3_IFIH1KO
UHi006-A-3
General
Cell Line |
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| hPSCreg name | UHi006-A-3 |
| Cite as: | UHi006-A-3 |
| Alternative name(s) |
HEL24.3_IFIH1KO
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 26th August 2024 |
| User feedback | |
Provider |
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| Generator |
University of Helsinki (UH)
Contact:
University of Helsinki (UH) |
| Owner | University of Helsinki (UH) |
| Distributors | |
| Derivation country | Finland |
External Databases |
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| BioSamples | SAMEA115941241 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Caucasian, White |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
SNP typing array
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA103885930 |
Ethics
Also have a look at the ethics information for the parental line
UHi006-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | CytoTuneTM-iPS Sendai Reprogramming Kit from Thermo-Fisher Scientific was used to create parental HEl24.3 hiPSC line |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution | The parent fibroblast cell-line obtained from the ATCC |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
UHi006-A.
|
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Reprogramming method |
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| Vector type | None |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 21 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| TRA 1-60 |
Yes |
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| SOX2 |
Yes |
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Morphology pictures
Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| CXCR4 |
Yes |
Morphology
Protocol or reference
Differentiation Protocol.pdf
Differentiation protocol reference
In vitro directed differentiation
Morphology
Protocol or reference
Differentiation Protocol.pdf
Differentiation protocol references
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46, XY
Passage number: 39
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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| Genetic modifications not related to a disease |
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