BIONi010-C-11

HNF1AP291fsinsC -/- 66-1

General#

Cell Line

hPSCreg Name BIONi010-C-11
Alternative name(s)
HNF1AP291fsinsC -/- 66-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 14th November 2017

Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors EBiSC (BIONi010-C-11 on EBiSC)
Derivation country Denmark

External Databases

BioSamples SAMEA104619382

General Information

Is the cell line available in principle? Available, without restrictions
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

Ethics#

We are currently revising the public data view for cell lines registered in hPSCreg. This section will be visible again in the next weeks.

hIPSC Derivation#

General

Primary cell type Fibroblast
Fibroblast in the OLS
NHDF-Ad-Der Fibroblasts
Primary cell line name
Primary cell line vendor

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Method documentation
HNF1A 66-1 flow.tif
Flow cytometry of undifferentiated cells

Microbiology / Virus Screening

Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
No

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic Modifications (other than for reprogramming)

Disease/phenotype related modifications
MODY
Synonyms
  • Maturity-onset diabetes of the young
Genetic modifications
HNF1A (target)
Isogenic modification
12q24.31
NM_000545.6:c.(c.872dup)
NP_000536.5:p.(P291fsinsC)
Homozygous
An additional cytocine has been introduced by CRISPR-Cas9 into a poly-C tract in both alleles of Exon 4 of HNF1A resulting in a out of frame mutation. Western blotting did not show any HNF1A protein in Pancreatic progenitor cells compared to the control line BIONi010-C.
Mutated
HNF1A 66-1 BIONi010-C.tif
Sequencing of HNF1A 66-1 and BIONi010-C showing a homozygous mutation in both alleles of HNF1A
Genetic modifications not related to a disease