BIONi010-C CD33 KO
BIONi010-C-9
General#
Cell Line |
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| hPSCreg Name | BIONi010-C-9 |
| Alternative name(s) |
BIONi010-C CD33 KO
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 9th August 2018 |
| User feedback | |
Provider |
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| Generator |
Bioneer (BION)
Contact:
Bioneer (BION) |
| Owner | Bioneer (BION) |
| Distributors | |
| Derivation country | Denmark |
External Databases |
|
| BioSamples | SAMEA4454012 |
| Cellosaurus | CVCL_LL40 |
| ECACC | 66540371 |
| EBiSC | BIONi010-C-9 |
General Information |
|
| Notes | No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23 |
| Projects | |
| Is the cell line available in principle? | Available, without restrictions |
| Subclone of | |
Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
| Disease associated phenotypes | no phenotypes |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unkown
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Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA3105780 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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The source cell information can be found in the parental cell line
BIONi010-C.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
No |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 18 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Morphology pictures
Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification#
| Disease/phenotype related modifications |
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