ApoE 3/3

General

Cell Line

hPSCreg name UKBi011-A-3
Cite as:
UKBi011-A-3 (RRID:CVCL_RX83)
Alternative name(s)
ApoE 3/3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UKBi011-A-1
(iLB-AD + ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-4
(ApoE 3/4)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-2
(ApoE 2/2)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A
(iLB-AD-169bm-s24)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A-1
(ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi072-A
(SFC058-03-01)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
STBCi072-B
(SFC058-03-02)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
STBCi072-C
(SFC058-03-03)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
STBCi006-A
(SFC140-04-01)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi256-A
(SFC144-03-02)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
BIONi010-C-3
(BIONi010-C ApoE KO #KO30 P30)
BIONi010-C-2
(BIONi010-C ApoE E3/E3 #H8 P32)
BIONi010-C-4
(BIONi010-C ApoE E4/E4 #B44 P27)
BIONi010-C-6
(BIONi010-C ApoE E2/E2)
BIONi037-A-2
(BIONi037-A ApoE2/2 #M10-7)
BIONi037-A-3
(BIONi037-A ApoE3/4 #P10-22)
BIONi037-A-4
(BIONi037-A ApoE4/4 #I10-53)
BIONi037-A-1
(16423 ApoE KO)
STBCi009-B
(SFC801-03-02)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi009-C
(SFC801-03-03)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi254-A
(SFC143-03-08)
Donor diseases:
Alzheimer disease
STBCi015-A
(SFC809-03-01)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi269-A
(BPC943-03-03)
Donor diseases:
Alzheimer disease
STBCi270-A
(BPC944-03-04)
Donor diseases:
Alzheimer disease
STBCi271-A
(BPC936-03-07)
Donor diseases:
Alzheimer disease
STBCi272-A
(BPC930-03-08)
Donor diseases:
Alzheimer disease
STBCi273-A
(BPC928-03-07)
Donor diseases:
Alzheimer disease
STBCi274-A
(BPC935-03-05)
Donor diseases:
Alzheimer disease
STBCi276-A
(BPC933-03-12)
Donor diseases:
Alzheimer disease
STBCi277-A
(BPC934-03-02)
Donor diseases:
Alzheimer disease
STBCi009-A
(SFC801-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi010-A
(SFC802-03-06)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi015-B
(SFC809-03-03)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi015-C
(SFC809-03-04)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi065-A
(SFC853-03-03)
Donor diseases:
Alzheimer disease
DRICUi011-A
(BS38A10002A)
Donor diseases:
late-onset Alzheimer's disease
DRICUi013-A
(SC45A10021A)
Donor diseases:
late-onset Alzheimer's disease
DRICUi006-A
(ADANG10496CA)
Donor diseases:
late-onset Alzheimer's disease
DRICUi007-A
(ADANG10605CA)
Donor diseases:
late-onset Alzheimer's disease
STBCi071-C
(SFC057-07-02)
Donor diseases:
Alzheimer disease
STBCi097-A
(SFC055-04-02)
Donor diseases:
Alzheimer disease
STBCi097-B
(SFC055-04-01)
Donor diseases:
Alzheimer disease
STBCi097-C
(SFC055-04-03)
Donor diseases:
Alzheimer disease
STBCi099-A
(SFC059-03-01)
Donor diseases:
Alzheimer disease
DRICUi012-A
(NT18A10014A)
Donor diseases:
late-onset Alzheimer's disease
STBCi013-A
(SFC807-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi014-A
(SFC808-03-03)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi061-A
(SFC850-03-01)
Donor's gene variants:
MAPT
Donor diseases:
Alzheimer disease
STBCi062-A
(SFC851-03-04)
Donor's gene variants:
MAPT
Donor diseases:
Alzheimer disease
DRICUi010-A
(BS38A10004A)
Donor diseases:
late-onset Alzheimer's disease
Last update 9th May 2022
Notes This line is part of a set of isogenic APOE lines based on the iPS cell line UKBi011-A. The set comprises the following APOE genotypes: • UKBi011-A-1 (APOE KO) • UKBi011-A-2 (APOE 2/2) • UKBi011-A-3 (APOE 3/3) • UKBi011-A-4 (APOE 3/4) • UKBi011-A (APOE 4/4) A DNA SNP array revealed no larger chromosomal aberrations to be reported. Other isogenic ApoE cell line cohorts are also available, generated from BIONi010-C, BIONi037-A and STBCi006-A.
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Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA104243176
Cellosaurus CVCL_RX83
Wikidata Q54990270

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Caucasia, German

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • AD
  • Alzheimer dementia
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimers dementia
  • Alzheimers disease
  • presenile and senile dementia
  • Alzheimer disease, familial
show more synonyms
Genetic variants
APOE (target)
19q13.3
NM_000041.4:c.388T>C
NP_000032.1:p.Cys130Arg
Homozygous
SCV000039748.4
The genotype at base position described by rs429358 is C/C, coding Arg, contributing to ApoE4/E4 variant
APOE (target)
19q13.32
NM_000041.3:c.526C
NP_000032.1:p.Arg176
Homozygous
The genotype at base position described by rs7412 is C/C, coding Arg, contributing to ApoE4/E4 variant.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104132641

Ethics

Also have a look at the ethics information for the parental line UKBi011-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

The source cell information can be found in the parental cell line UKBi011-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 18 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 28

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • AD
  • Alzheimer dementia
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimers dementia
  • Alzheimers disease
  • presenile and senile dementia
  • Alzheimer disease, familial
show more synonyms
Genetic modifications
APOE (target)
Isogenic modification
19q13.32
NM_000041.4:c.388C>T
NP_000032.1:p.Arg130Cys
Homozygous
UKBi011-A-3 ApoE3/E3 is an isogenic mutant of UKBi011-A (ApoE4/E4); The genotype at base position described by rs429358 has been modified in the subclone from C/C to T/T, which changes aa from Arg to Cys. Together with C/C at rs7412 these define a ApoE3/E3 genotype in the subclone. Please see chromatogram for confirmation of the base changes in the subclone.
Mutated