SFC057-07-02

General

Cell Line

hPSCreg name STBCi071-C
Cite as:
STBCi071-C (RRID:CVCL_RC97)
Alternative name(s)
SFC057-07-02
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
STBCi071-A
(SFC057-07-03)
Donor diseases:
Alzheimer disease
STBCi071-B
(SFC057-07-01)
Donor diseases:
Alzheimer disease
UKBi011-A-1
(iLB-AD + ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-2
(ApoE 2/2)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-3
(ApoE 3/3)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-4
(ApoE 3/4)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A-1
(ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A-3
(ApoE 3/3)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A-4
(ApoE 3/4)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UMi042-A-1
(408737 CB9)
Donor diseases:
Alzheimer's Disease
STBCi009-A
(SFC801-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi009-B
(SFC801-03-02)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi009-C
(SFC801-03-03)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi010-A
(SFC802-03-06)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi015-A
(SFC809-03-01)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi015-B
(SFC809-03-03)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi015-C
(SFC809-03-04)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi047-A
(SFC043-03-01)
Donor diseases:
Alzheimer disease
STBCi051-A
(SFC056-04-03)
Donor diseases:
Alzheimer disease
STBCi047-B
(SFC043-03-13)
Donor diseases:
Alzheimer disease
STBCi051-B
(SFC056-04-01)
Donor diseases:
Alzheimer disease
STBCi051-C
(SFC056-04-02)
Donor diseases:
Alzheimer disease
STBCi065-A
(SFC853-03-03)
Donor diseases:
Alzheimer disease
STBCi072-A
(SFC058-03-01)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
STBCi072-B
(SFC058-03-02)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
STBCi072-C
(SFC058-03-03)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
STBCi077-A
(SFC136-03-02)
Donor diseases:
Alzheimer disease
STBCi077-B
(SFC136-03-03)
Donor diseases:
Alzheimer disease
STBCi077-C
(SFC136-03-01)
Donor diseases:
Alzheimer disease
STBCi097-A
(SFC055-04-02)
Donor diseases:
Alzheimer disease
STBCi097-B
(SFC055-04-01)
Donor diseases:
Alzheimer disease
STBCi097-C
(SFC055-04-03)
Donor diseases:
Alzheimer disease
STBCi099-A
(SFC059-03-01)
Donor diseases:
Alzheimer disease
STBCi099-B
(SFC059-03-02)
Donor diseases:
Alzheimer disease
STBCi134-A
(SFC019-03-01)
Donor diseases:
Alzheimer disease
STBCi254-A
(SFC143-03-08)
Donor diseases:
Alzheimer disease
IRMBi001-A
(AD-PS1 hiPSC)
Donor diseases:
obsolete_Alzheimer's disease
ZZUi009-A
(ZZU-iPS-AD-PS1-001)
Donor diseases:
obsolete_Alzheimer's disease
STBCi011-A
(SFC803-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi011-B
(SFC803-03-02)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi011-C
(SFC803-03-08)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi012-A
(SFC805-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi012-B
(SFC805-03-02)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi012-C
(SFC805-03-03)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi013-A
(SFC807-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi013-B
(SFC807-03-04)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi014-A
(SFC808-03-03)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi014-B
(SFC808-03-04)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
Last update 23rd March 2023
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Provider

Generator StemBANCC (STBC)
Distributors

External Databases

BioSamples SAMEA104493896
Cellosaurus CVCL_RC97
Wikidata Q54956574

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 75-79

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • AD
  • Alzheimer dementia
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimers dementia
  • Alzheimers disease
  • presenile and senile dementia
  • Alzheimer disease, familial
show more synonyms
Genetic variants

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493812

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink zameel.cader@ndcn.ox.ac.uk
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? NRES Committee South Central - Hampshire A
Approval number 13/SC/0179
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? NRES Committee South Central - Hampshire A
Approval number 13/SC/0179
Do you have obligations to third parties in regard to the use of the cell line? Yes
Please describe: DNA sequencing can only be performed for research into donors specified condition. Material shall not be sold, transplanted into any human being or used to create egg or sperm cells (gametes) or embryos. The material shall not be used for direct exploitation. For the purposes of this, Direct exploitation means to develop for commericalization or to commercialize the Material.
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 75-79

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
CXCR4
Yes
Gata6
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
MIXL1
Yes
DCN
Yes
VIM
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
NEUROD1
Yes
HES5
Yes
PAX6
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 12

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
minor Chromosome 7 lower copy number variation which is not present in parental fibroblasts
Passage number: 12
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)