STBCi006-A-3

ApoE 3/3

General#

Cell Line

hPSCreg Name STBCi006-A-3
Alternative name(s)
ApoE 3/3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 18th September 2019
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Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors

External Databases

BioSamples SAMEA104243172
EBiSC STBCi006-A-3
ECACC 66540615
Cellosaurus CVCL_RX82

General Information

Notes This line is part of a set of isogenic APOE lines based on the iPS cell line STBCi006-A. The set comprises the following APOE genotypes: • STBCi006-A-3 (APOE 3/3) • STBCi006-A-4 (APOE 3/4) • STBCi006-A (APOE 4/4) • STBCi006-A-1 (APOE KO) A DNA SNP array revealed a duplication of 2 MB on chromosome 20q, with significant genes involved (refer to “SNP typing array” in the Genotyping section for more information). This duplication was found in all isogenic lines generated from STBCi006-A. Other isogenic ApoE cell line cohorts are also available, generated from BIONi010-C, BIONi37-A and UKBi011-A.
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Alzheimer's disease
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Disease, Alzheimer
  • Dementia in Alzheimer's disease, unspecified (disorder)
  • Presenile Alzheimer Dementia
  • ALZHEIMERS DIS
  • Alzheimers
  • DAT - Dementia Alzheimer's type
  • Dementia in Alzheimer's disease
  • Alzheimer's disease, NOS
  • Alzheimer Dementia, Presenile
  • Dementia, Alzheimer Type
  • Alzheimer Dementia
  • Alzheimer's Dementia
  • Alzheimer's
  • Dementia, Presenile
  • [X]Dementia in Alzheimer's disease (disorder)
  • ALZHEIMER DIS
  • AD
  • [X]Dementia in Alzheimer's disease
  • AD - Alzheimer's disease
  • Disease, Alzheimer's
  • Alzheimer Disease
  • Dementia, Presenile Alzheimer
  • Alzheimer Type Dementia
  • Dementia in Alzheimer's disease (disorder)
  • Alzheimer's disease (disorder)
  • Alzheimers Dementia
  • Dementia of the Alzheimer's type
  • sporadic Alzheimer's disease
  • Alzheimers disease
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimer dementia
  • Alzheimers dementia
show more synonyms
Genetic variants
ApoE (target)
19q13.3
NM_000041.4:c.388T>C
NP_000032.1:p.Cys130Arg
Homozygous
SCV000039748.4
The genotype at base position described by rs429358 is C/C, coding Arg, contributing to ApoE4/E4 variant
ApoE (target)
19q13.32
NM_000041.3:c.526C
NP_000032.1:p.Arg176
Homozygous
The genotype at base position described by rs7412 is C/C, coding Arg, contributing to ApoE4/E4 variant.

External Databases (Donor)

BioSamples SAMEA104133819

Ethics#

Also have a look at the ethics information for the parental line STBCi006-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation#

General

The source cell information can be found in the parental cell line STBCi006-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 18 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array
SNP array done by Life&Brain

Genetic Modification#

Disease/phenotype related modifications
Alzheimer's disease
Synonyms
  • Disease, Alzheimer
  • Dementia in Alzheimer's disease, unspecified (disorder)
  • Presenile Alzheimer Dementia
  • ALZHEIMERS DIS
  • Alzheimers
  • DAT - Dementia Alzheimer's type
  • Dementia in Alzheimer's disease
  • Alzheimer's disease, NOS
  • Alzheimer Dementia, Presenile
  • Dementia, Alzheimer Type
  • Alzheimer Dementia
  • Alzheimer's Dementia
  • Alzheimer's
  • Dementia, Presenile
  • [X]Dementia in Alzheimer's disease (disorder)
  • ALZHEIMER DIS
  • AD
  • [X]Dementia in Alzheimer's disease
  • AD - Alzheimer's disease
  • Disease, Alzheimer's
  • Alzheimer Disease
  • Dementia, Presenile Alzheimer
  • Alzheimer Type Dementia
  • Dementia in Alzheimer's disease (disorder)
  • Alzheimer's disease (disorder)
  • Alzheimers Dementia
  • Dementia of the Alzheimer's type
  • sporadic Alzheimer's disease
  • Alzheimers disease
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimer dementia
  • Alzheimers dementia
show more synonyms
Genetic modifications
ApoE (target)
Isogenic modification
19q13.32
NM_000041.4:c.388C>T
NP_000032.1:p.Arg130Cys
Homozygous
STBCi006-A-3 ApoE3/E3 is an isogenic mutant of STBCi006-A (ApoE4/E4); The genotype at base position described by rs429358 has been modified in the subclone from C/C to T/T, which changes aa from Arg to Cys. Together with a C/C genotype at rs7412 these define a ApoE3/E3 genotype in the subclone. Please see chromatogram for confirmation of the base changes in the subclone.
Changed