ApoE 3/3

General

Cell Line

hPSCreg name STBCi006-A-3
Cite as:
STBCi006-A-3 (RRID:CVCL_RX82)
Alternative name(s)
ApoE 3/3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
STBCi006-A-1
(ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A
(SFC140-04-01)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-1
(iLB-AD + ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-3
(ApoE 3/3)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-4
(ApoE 3/4)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-2
(ApoE 2/2)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi256-A
(SFC144-03-02)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
UKBi011-A
(iLB-AD-169bm-s24)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi072-A
(SFC058-03-01)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
STBCi072-B
(SFC058-03-02)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
STBCi072-C
(SFC058-03-03)
Donor's gene variants:
APOE
Donor diseases:
Alzheimer disease
BIONi037-A-2
(BIONi037-A ApoE2/2 #M10-7)
BIONi037-A-3
(BIONi037-A ApoE3/4 #P10-22)
BIONi037-A-4
(BIONi037-A ApoE4/4 #I10-53)
BIONi037-A-1
(16423 ApoE KO)
BIONi010-C-3
(BIONi010-C ApoE KO #KO30 P30)
BIONi010-C-2
(BIONi010-C ApoE E3/E3 #H8 P32)
BIONi010-C-4
(BIONi010-C ApoE E4/E4 #B44 P27)
BIONi010-C-6
(BIONi010-C ApoE E2/E2)
STBCi013-A
(SFC807-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi014-A
(SFC808-03-03)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi061-A
(SFC850-03-01)
Donor's gene variants:
MAPT
Donor diseases:
Alzheimer disease
STBCi062-A
(SFC851-03-04)
Donor's gene variants:
MAPT
Donor diseases:
Alzheimer disease
DRICUi010-A
(BS38A10004A)
Donor diseases:
late-onset Alzheimer's disease
DRICUi012-A
(NT18A10014A)
Donor diseases:
late-onset Alzheimer's disease
STBCi011-A
(SFC803-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi011-B
(SFC803-03-02)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi011-C
(SFC803-03-08)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi012-A
(SFC805-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi012-B
(SFC805-03-02)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi012-C
(SFC805-03-03)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi013-B
(SFC807-03-04)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi014-B
(SFC808-03-04)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi014-C
(SFC808-03-06)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi264-A
(SFC142-03-01)
Donor diseases:
Alzheimer disease
STBCi275-A
(BPC937-03-06)
Donor diseases:
Alzheimer disease
STBCi284-A
(BPC939-03-01)
Donor diseases:
Alzheimer disease
STBCi285-A
(BPC940-03-08)
Donor diseases:
Alzheimer disease
STBCi300-A
(BPC932-03-03)
Donor diseases:
Alzheimer disease
STBCi301-A
(BPC949-04-11)
Donor diseases:
Alzheimer disease
STBCi302-A
(BPC945-03-01)
Donor diseases:
Alzheimer disease
STBCi009-A
(SFC801-03-01)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi010-A
(SFC802-03-06)
Donor's gene variants:
PSEN1
Donor diseases:
Alzheimer disease
STBCi015-B
(SFC809-03-03)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi015-C
(SFC809-03-04)
Donor's gene variants:
APP
Donor diseases:
Alzheimer disease
STBCi065-A
(SFC853-03-03)
Donor diseases:
Alzheimer disease
DRICUi011-A
(BS38A10002A)
Donor diseases:
late-onset Alzheimer's disease
DRICUi013-A
(SC45A10021A)
Donor diseases:
late-onset Alzheimer's disease
DRICUi006-A
(ADANG10496CA)
Donor diseases:
late-onset Alzheimer's disease
DRICUi007-A
(ADANG10605CA)
Donor diseases:
late-onset Alzheimer's disease
Last update 9th May 2022
Notes This line is part of a set of isogenic APOE lines based on the iPS cell line STBCi006-A. The set comprises the following APOE genotypes: • STBCi006-A-3 (APOE 3/3) • STBCi006-A-4 (APOE 3/4) • STBCi006-A (APOE 4/4) • STBCi006-A-1 (APOE KO) A DNA SNP array revealed a duplication of 2 MB on chromosome 20q, with significant genes involved (refer to “SNP typing array” in the Genotyping section for more information). This duplication was found in all isogenic lines generated from STBCi006-A. Other isogenic ApoE cell line cohorts are also available, generated from BIONi010-C, BIONi37-A and UKBi011-A.
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Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors

External Databases

BioSamples SAMEA104243172
EBiSC STBCi006-A-3
Cellosaurus CVCL_RX82
Wikidata Q54956334

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed
Subclone of

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • AD
  • Alzheimer dementia
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimers dementia
  • Alzheimers disease
  • presenile and senile dementia
  • Alzheimer disease, familial
show more synonyms
Genetic variants
APOE (target)
19q13.3
NM_000041.4:c.388T>C
NP_000032.1:p.Cys130Arg
Homozygous
SCV000039748.4
The genotype at base position described by rs429358 is C/C, coding Arg, contributing to ApoE4/E4 variant
APOE (target)
19q13.32
NM_000041.3:c.526C
NP_000032.1:p.Arg176
Homozygous
The genotype at base position described by rs7412 is C/C, coding Arg, contributing to ApoE4/E4 variant.

External Databases (Donor)

BioSamples SAMEA104133819

Ethics

Also have a look at the ethics information for the parental line STBCi006-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

The source cell information can be found in the parental cell line STBCi006-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 18 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 28
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array
SNP array done by Life&Brain

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • AD
  • Alzheimer dementia
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimers dementia
  • Alzheimers disease
  • presenile and senile dementia
  • Alzheimer disease, familial
show more synonyms
Genetic modifications
APOE (target)
Isogenic modification
19q13.32
NM_000041.4:c.388C>T
NP_000032.1:p.Arg130Cys
Homozygous
STBCi006-A-3 ApoE3/E3 is an isogenic mutant of STBCi006-A (ApoE4/E4); The genotype at base position described by rs429358 has been modified in the subclone from C/C to T/T, which changes aa from Arg to Cys. Together with a C/C genotype at rs7412 these define a ApoE3/E3 genotype in the subclone. Please see chromatogram for confirmation of the base changes in the subclone.
Changed