BIONi037-A-2

BIONi037-A ApoE2/2 #M10-7

General#

Cell Line

hPSCreg Name BIONi037-A-2
Alternative name(s)
BIONi037-A ApoE2/2 #M10-7
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 5th June 2019
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Provider

Generator Bioneer (BSC)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA104235612
EBiSC BIONi037-A-2
ECACC 66540604
Cellosaurus CVCL_RF92

General Information

Notes This line is part of a set of isogenic APOE lines based on the iPS cell line BIONi037-A. The set comprises the following APOE genotypes: • BIONi037-A-1 (APOE KO) • BIONi037-A-2 (APOE 2/2) • BIONi037-A (APOE 3/3) • BIONi037-A-3 (APOE 3/4) • BIONi037-A-4 (APOE 4/4) A DNA SNP array revealed no larger chromosomal aberrations to be reported. Other isogenic ApoE cell line cohorts are also available, generated from BIONi010-C, UKBi011-A and STBci006-A.
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104026331

Ethics#

Also have a look at the ethics information for the parental line BIONi037-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line BIONi037-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Morphology pictures

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Alzheimer's disease
Synonyms
  • Disease, Alzheimer
  • Dementia in Alzheimer's disease, unspecified (disorder)
  • Presenile Alzheimer Dementia
  • ALZHEIMERS DIS
  • Alzheimers
  • DAT - Dementia Alzheimer's type
  • Dementia in Alzheimer's disease
  • Alzheimer's disease, NOS
  • Alzheimer Dementia, Presenile
  • Dementia, Alzheimer Type
  • Alzheimer Dementia
  • Alzheimer's Dementia
  • Alzheimer's
  • Dementia, Presenile
  • [X]Dementia in Alzheimer's disease (disorder)
  • ALZHEIMER DIS
  • AD
  • [X]Dementia in Alzheimer's disease
  • AD - Alzheimer's disease
  • Disease, Alzheimer's
  • Alzheimer Disease
  • Dementia, Presenile Alzheimer
  • Alzheimer Type Dementia
  • Dementia in Alzheimer's disease (disorder)
  • Alzheimer's disease (disorder)
  • Alzheimers Dementia
  • Dementia of the Alzheimer's type
  • sporadic Alzheimer's disease
  • Alzheimers disease
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimer dementia
  • Alzheimers dementia
show more synonyms
Genetic modifications
ApoE (target)
Isogenic modification
19q13.32
NM_000041.3:c.526C>T
NP_000032.1:p.Arg176Cys
Homozygous
BIONi037-A-2 ApoE2/E2 is an isogenic mutant of BIONi037-A (ApoE3/E3); The genotype at base position described by rs7412 has been modified in the subclone from C/C to T/T, which changes aa from Arg to Cys. This then defines a ApoE2/E2 genotype in the subclone. Please see chromatogram for confirmation of the base changes in the subclone.
Changed