BIONi037-A-2

BIONi037-A ApoE2/2 #M10-7

General

Cell Line

hPSCreg name BIONi037-A-2
Cite as:
BIONi037-A-2 (RRID:CVCL_RF92)
Alternative name(s)
BIONi037-A ApoE2/2 #M10-7
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
BIONi037-A-3
(BIONi037-A ApoE3/4 #P10-22)
BIONi037-A-4
(BIONi037-A ApoE4/4 #I10-53)
BIONi037-A-1
(16423 ApoE KO)
STBCi006-A-1
(ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A-3
(ApoE 3/3)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A-4
(ApoE 3/4)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UMi042-A-1
(408737 CB9)
Donor diseases:
Alzheimer's Disease
BIONi037-A
(16423 ApoE3/3 (control line))
UKBi011-A-3
(ApoE 3/3)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-4
(ApoE 3/4)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-1
(iLB-AD + ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-2
(ApoE 2/2)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
BIONi010-C-2
(BIONi010-C ApoE E3/E3 #H8 P32)
BIONi010-C-3
(BIONi010-C ApoE KO #KO30 P30)
BIONi010-C-6
(BIONi010-C ApoE E2/E2)
BIONi010-C-4
(BIONi010-C ApoE E4/E4 #B44 P27)
SIGi001-A-13
(iPSC0028 – MonoAllelic MAPT_Ex10+16T/Clone 1D01-11)
STBCi026-A-1
(SFC840-03-03 LRRK2-/-D10)
STBCi026-A-3
(SFC840-03-03 LRRK2 WT/R1441C H3)
GIBHi002-A-1
(C5-PARK2-KO)
STBCi026-A-2
(SFC840-03-03 LRRK2-/-C11)
Last update 31st January 2024
Notes This line is part of a set of isogenic APOE lines based on the iPS cell line BIONi037-A. The set comprises the following APOE genotypes: • BIONi037-A-1 (APOE KO) • BIONi037-A-2 (APOE 2/2) • BIONi037-A (APOE 3/3) • BIONi037-A-3 (APOE 3/4) • BIONi037-A-4 (APOE 4/4) A DNA SNP array revealed no larger chromosomal aberrations to be reported. Other isogenic ApoE cell line cohorts are also available, generated from BIONi010-C, UKBi011-A and STBci006-A.
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Provider

Generator Bioneer (BSC)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA104235612
EBiSC BIONi037-A-2
Cellosaurus CVCL_RF92
Wikidata Q54796878

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Sanger sequencing
The donor of this line carries the ApoE3/E3 allele.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104026331

Ethics

Also have a look at the ethics information for the parental line BIONi037-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line BIONi037-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Medium Essential 8™

Characterisation

Analysis of Undifferentiated Cells
Morphology pictures

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 23
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • AD
  • Alzheimer dementia
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimers dementia
  • Alzheimers disease
  • presenile and senile dementia
  • Alzheimer disease, familial
show more synonyms
Genetic modifications
APOE (target)
Isogenic modification
19q13.32
NM_000041.3:c.526C>T
NP_000032.1:p.Arg176Cys
Homozygous
BIONi037-A-2 ApoE2/E2 is an isogenic mutant of BIONi037-A (ApoE3/E3); The genotype at base position described by rs7412 has been modified in the subclone from C/C to T/T, which changes aa from Arg to Cys. This then defines a ApoE2/E2 genotype in the subclone. Please see chromatogram for confirmation of the base changes in the subclone.
Changed