16423 ApoE3/3 (control line)

General#

Cell Line

hPSCreg Name BIONi037-A
Alternative name(s)
16423 ApoE3/3 (control line)
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 21st September 2017

Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors EBiSC (BIONi037-A on EBiSC)
Derivation country Denmark

External Databases

BioSamples SAMEA104026330

General Information

Publications
Is the cell line available in principle? Available, without restrictions
Subclones

Donor Information#

General Donor Information

Sex female
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unkown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104026331

Ethics#

We are currently revising the public data view for cell lines registered in hPSCreg. This section will be visible again in the next weeks.

hIPSC Derivation#

General

Primary cell type Fibroblast of dermis
Fibroblast of dermis in the OLS
Primary cell line name
Primary cell line vendor
Developmental stage primary cell Adult
Age of donor at collection 75-79
Location of primary tissue procurement Tübingen, Germany
Date of collection 2010-09-01
Passage number reprogrammed 18

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
No
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 18 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Tuj1, SMA and AFP have been analysed after spontaneous differentiation as embryoic bodies. See publication: https://doi.org/10.1016/j.scr.2016.09.019
Method documentation
Differentiation Potency

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
No

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic Modifications (other than for reprogramming)