BIONi037-A-4

BIONi037-A ApoE4/4 #I10-53

General

Cell Line

hPSCreg Name
BIONi037-A-4
Alternative name(s)
BIONi037-A ApoE4/4 #I10-53
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2022
Notes This line is part of a set of isogenic APOE lines based on the iPS cell line BIONi037-A. The set comprises the following APOE genotypes: • BIONi037-A-1 (APOE KO) • BIONi037-A-2 (APOE 2/2) • BIONi037-A (APOE 3/3) • BIONi037-A-3 (APOE 3/4) • BIONi037-A-4 (APOE 4/4) A DNA SNP array revealed no larger chromosomal aberrations to be reported. Other isogenic ApoE cell line cohorts are also available, generated from BIONi010-C, UKBi011-A and STBci006-A.
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Bioneer (BSC)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA104235614
EBiSC BIONi037-A-4
ECACC 66540606
Cellosaurus CVCL_RF94
CLO CLO_0102749
Wikidata Q54796882

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Sanger sequencing
The donor of this line carries the ApoE3/E3 allele.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104026331

Ethics

Also have a look at the ethics information for the parental line BIONi037-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line BIONi037-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
No
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions

Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4
Yes
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Alzheimer disease
Genetic modifications
APOE (target)
Isogenic modification
19q13.32
NM_000041.4:c.388T>C
NP_000032.1:p.Cys130Arg
Homozygous
BIONi037-A-4 ApoE4/E4 is an isogenic mutant of BIONi037-A (ApoE3/E3); The genotype at base position described by rs429358 has been modified in the subclone from T/T to C/C, which changes aa from Cys to Arg; The genotype of base position described by rs7412 in both parent and subclone is C/C, which is an Arg. Together these define a ApoE4/E4 genotype in the subclone. Please see chromatogram for confirmation of the base changes in the subclone.
Changed