BIHi043-A-SORL_SNP_Cl-2
HMGUi001-A-44
General
Cell Line |
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| hPSCreg name | HMGUi001-A-44 |
| Cite as: | HMGUi001-A-44 |
| Alternative name(s) |
BIHi043-A-SORL_SNP_Cl-2
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
HMGUi001-A (XM001, BIHi043-A) HMGUi001-A-42 (NEUROD2 nVenus/nVenus iPSCs) HMGUi001-A-1 (hINS-T2A-H2B-Cherry (+/-), HMGUi001-A-1) HMGUi001-A-54 (ARX-T2A-H2B-CFP x C-PEP-mCherry -hiPSC, ARX-CFPxC-PEP-mCherry-hiPSC, ARXCFP/CFP x C-PEPmCherry/+ hiPSC) HMGUi001-A-22 (NCS1-KO Clone 19) HMGUi001-A-55 (Flattop-T2A-H2B-Venus-hiPSC) HMGUi001-A-56 (Flattop-T2A-H2B-VenusxC-Peptide-mCherry-hiPSC) HMGUi001-A-43 (hINS-T2A-H2B-Cherry (-/-), hiPSC-INS-T2A-H2B-Cherry reporter, INSCherry/Cherry) HMGUi001-A-18 (BIHi043-A SORL1_KO Cl B1) HMGUi001-A-19 (BIHi043-A SORL1_KO Cl F2) HMGUi001-A-4 (hiPSC-ARX-T2A-H2B-CFP-Flag) HMGUi001-A-8 (C-PEP-mCherry-hiPSC) HMGUi001-A-61 (SYT13-KOnVenus/nVenus-hiPSC) HMGUi001-A-46 (ARX-CFP/PAX4-mCherry, ARX-T2A-H2B-CFP/H2B-mCherry-RGSHis-T2A-PAX4, ARXnCFP/nCFP/PAX4mCherry/mCherry) HMGUi001-A-5 (ΔINK4 T2D risk region hiPSC) SIGi001-A-13 (iPSC0028 – MonoAllelic MAPT_Ex10+16T/Clone 1D01-11) BIONi037-A-2 (BIONi037-A ApoE2/2 #M10-7) BIONi037-A-3 (BIONi037-A ApoE3/4 #P10-22) BIONi037-A-4 (BIONi037-A ApoE4/4 #I10-53) GEMi011-A-1 (GN-JY02-02-011(PSEN1-exon9-KO)) BIONi037-A-1 (16423 ApoE KO) WIBRe001-A-12 (WIBR3_PINK1_Q129X_E2-2) WIBRe001-A-13 (WIBR3_PINK1_Q129X_E7-1) WIBRe001-A-15 (WIBR3_DNAJC6_c.801-2 A>G+FS/c.801-2 A>G_G12-2) WIBRe001-A-16 (WIBR3_DNAJC6_FS/FS_H10-1) WIBRe001-A-17 (WIBR3_GBA1_IVS2_G2E) WIBRe001-A-18 (WIBR3_GBA1_IVS2_E10B) WIBRe001-A-58 (WIBR3_VPS13C_FS_Homo_H3-1) WIBRe001-A-59 (WIBR3_VPS13C_A444P_Het_E12-1) WIBRe001-A-60 (WIBR3_VPS13C_A444P_Homo_C8-2) WIBRe001-A-5 (WIBR3_PRKN_X3DEL_F2-5) WIBRe001-A-6 (WIBR3_PRKN_X3DEL_H2-2) WIBRe001-A-7 (WIBR3_SNCA_A30P_A2-3) WIBRe001-A-8 (WIBR3_SNCA_A30P_E1-3) WIBRe001-A-9 (WIBR3_FBXO7_R498X_A7-1) WIBRe001-A-10 (WIBR3_FBXO7_FS_A3-1) WIBRe001-A-11 (WIBR3_PINK1_Q129X_C4-1) |
| Last update | 6th November 2024 |
| User feedback | |
Provider |
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| Generator | Berlin Institute of Health (BIH) |
External Databases |
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| BioSamples | SAMEA116351595 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Family history | no |
| Is the medical history available upon request? | no |
| Is clinical information available? | no |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA5696688 |
Ethics
Also have a look at the ethics information for the parental line
HMGUi001-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
HMGUi001-A.
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Reprogramming method |
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Morphology pictures
Morphology.tif
Morpholgy after thawing the bank
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Certificate of Analysis |
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| Is there a certificate of analysis available? |
Yes
Passage:
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Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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