General#

Cell Line

hPSCreg Name BIHi005-A-5
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 5th February 2018

Provider

Generator Berlin Institute of Health (BIH)

External Databases

BioSamples SAMEA1009819

General Information

Notes SORL1 KO in BIHi005-A
Is the cell line available in principle? Available with restrictions: Available for non-commercial research
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity asian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history Mutation in the LMNA gene

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104012190

Ethics#

We are currently revising the public data view for cell lines registered in hPSCreg. This section will be visible again in the next weeks.

hIPSC Derivation#

General

The source cell information can be found in the parental cell line BIHi005-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
Immunostaining

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
SOX2
Yes
Differentiation Potency

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Genetic Modifications (other than for reprogramming)
Yes
Disease/phenotype related modifications
Alzheimers disease
Synonyms
  • Disease, Alzheimer
  • Dementia in Alzheimer's disease, unspecified (disorder)
  • Presenile Alzheimer Dementia
  • ALZHEIMERS DIS
  • Alzheimers
  • DAT - Dementia Alzheimer's type
  • Dementia in Alzheimer's disease
  • Alzheimer's disease, NOS
  • Alzheimer Dementia, Presenile
  • Dementia, Alzheimer Type
  • Alzheimer Dementia
  • Alzheimer's Dementia
  • Alzheimer's
  • Dementia, Presenile
  • [X]Dementia in Alzheimer's disease (disorder)
  • ALZHEIMER DIS
  • AD
  • [X]Dementia in Alzheimer's disease
  • AD - Alzheimer's disease
  • Disease, Alzheimer's
  • Alzheimer Disease
  • Dementia, Presenile Alzheimer
  • Alzheimer Type Dementia
  • Dementia in Alzheimer's disease (disorder)
  • Alzheimer's disease (disorder)
  • Alzheimers Dementia
  • Dementia of the Alzheimer's type
  • sporadic Alzheimer's disease
show more synonyms
Genetic modifications
SORL1 (target)
Gene knock-out
11q24.1
TALEN