BIONi010-C-17

BIONi010-C TREM2 KO

General#

Cell Line

hPSCreg Name BIONi010-C-17
Alternative name(s)
BIONi010-C TREM2 KO
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 12th August 2019
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Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA104386270
EBiSC BIONi010-C-17
ECACC 66540632
Cellosaurus CVCL_RM88

General Information

Notes No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

Ethics#

Also have a look at the ethics information for the parental line BIONi010-C .
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line BIONi010-C.

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
SSEA-1
No
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
TREM2 KO cl. 24-54 D1 thaw after banking.tif
Phase contrast image 1 day after thawing
Method documentation
06-Sep-2017-Layout.tiff
Flow Cytometry with pluripotency markers
qPCR BIONi010-C-17.tif
qPCR with pluripotency markers
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Protocol or reference
BIONi010-C-17 endo D5.tif
Flow cytometry of in vitro differentiation to endoderm
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Protocol or reference
BIONi010-C-17 meso D5.tif
Flow cytometry of in vitro differentiation to mesoderm
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Protocol or reference
BIONi010-C-17 ecto D5.tif
Flow cytometry of in vitro differentiation to ectoderm

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Alzheimer's disease
Synonyms
  • Disease, Alzheimer
  • Dementia in Alzheimer's disease, unspecified (disorder)
  • Presenile Alzheimer Dementia
  • ALZHEIMERS DIS
  • Alzheimers
  • DAT - Dementia Alzheimer's type
  • Dementia in Alzheimer's disease
  • Alzheimer's disease, NOS
  • Alzheimer Dementia, Presenile
  • Dementia, Alzheimer Type
  • Alzheimer Dementia
  • Alzheimer's Dementia
  • Alzheimer's
  • Dementia, Presenile
  • [X]Dementia in Alzheimer's disease (disorder)
  • ALZHEIMER DIS
  • AD
  • [X]Dementia in Alzheimer's disease
  • AD - Alzheimer's disease
  • Disease, Alzheimer's
  • Alzheimer Disease
  • Dementia, Presenile Alzheimer
  • Alzheimer Type Dementia
  • Dementia in Alzheimer's disease (disorder)
  • Alzheimer's disease (disorder)
  • Alzheimers Dementia
  • Dementia of the Alzheimer's type
  • sporadic Alzheimer's disease
  • Alzheimers disease
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimer dementia
  • Alzheimers dementia
show more synonyms
Genetic modifications
TREM2 (target)
Gene knock-out
6p21.1
KO of exon 1 of TREM2 Transcript variant 1 NM_018965
CRISPR-associated (CRISPR/Cas) System
TREM2 KO BIONI010-C-17.pdf
Sequencing of KO site and verification of single clone