BIONi010-C TREM2 KO
BIONi010-C-17
General
Donor Information
General Donor Information |
|
Sex | male |
Ethnicity | Black or African-American |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
Karyotyping (Donor) |
|
Has the donor karyotype been analysed? |
Unknown
|
Other Genotyping (Donor) |
|
Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
Other cell lines of this donor | |
External Databases (Donor) |
|
BioSamples | SAMEA3105780 |
Ethics
Also have a look at the ethics information for the parental line
BIONi010-C
.
Is there an MTA available for the cell line? | Yes |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
|
The source cell information can be found in the parental cell line
BIONi010-C.
|
|
Reprogramming method |
|
Vector type | Non-integrating |
Vector | Episomal |
Vector free reprogramming |
|
Other |
|
Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
SOX2 |
Yes |
|
||||
POU5F1 (OCT-4) |
Yes |
|
||||
SSEA-3 |
Yes |
|
||||
SSEA-4 |
Yes |
|
||||
SSEA-1 |
No |
|
Score:
Marker | Present | Absent |
mCpG | ||
OCT4 |
Morphology pictures
TREM2 KO cl. 24-54 D1 thaw after banking.tif
Phase contrast image 1 day after thawing
Method documentation
06-Sep-2017-Layout.tiff
Flow Cytometry with pluripotency markers
qPCR BIONi010-C-17.tif
qPCR with pluripotency markers
Differentiation Potency
In vitro directed differentiation
Morphology
Protocol or reference
BIONi010-C-17 endo D5.tif
Flow cytometry of in vitro differentiation to endoderm
In vitro directed differentiation
Morphology
Protocol or reference
BIONi010-C-17 meso D5.tif
Flow cytometry of in vitro differentiation to mesoderm
In vitro directed differentiation
Morphology
Protocol or reference
BIONi010-C-17 ecto D5.tif
Flow cytometry of in vitro differentiation to ectoderm
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
Genetic Modification
Disease/phenotype related modifications |
|
Login to share your feedback, experiences or results with the research community.