FUS-P525L HOM 2D2
UCSFi001-A-75
General
Cell Line |
|
| hPSCreg name | UCSFi001-A-75 |
| Cite as: | UCSFi001-A-75 (RRID:CVCL_D0NL) |
| Alternative name(s) |
FUS-P525L HOM 2D2
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
UCSFi001-A-74 (FUS-P525L HET 2D1) UCSFi001-A-72 (FUS-R521G HET 2B10, LSUHSi004-A-72) UCSFi001-A (wtc11, Wtc11, WTC, WTC11, GM25256) WTSIi018-B-24 (Kolf 2.1 J (FUS R495X WT/SNV)) WTSIi018-B-25 (Kolf 2.1 J (FUS R495X SNV/SNV)) WTSIi018-B-26 (Kolf 2.1 J (FUS R521H WT/SNV)) WTSIi018-B-27 (Kolf 2.1 J (FUS R521H SNV/SNV)) UCSFi001-A-73 (FUS-R521G HOM 1D9) UCSFi001-A-49 (YH653-MUT-1C8-HET, DYT1-HET-1C8) UCSFi001-A-50 (YH653-MUT-H6-HET, DYT1-HET-H6) UCSFi001-A-51 (YH653-MUT-2F2-HOMO, DYT1-HOMO-2F2) UCSFi001-A-64 (P633L RBM20 iPSCs) UCSFi001-A-2 (AICS-0012-105) UCSFi001-A-3 (AICS-0016-184) UCSFi001-A-4 (AICS-0075-085) UCSFi001-A-63 (AICS-0082-001) UCSFi001-A-5 (AICS-0011-027) UCSFi001-A-6 (AICS-0013-210) UCSFi001-A-66 (AICS-0114-032) UCSFi001-A-67 (AICS-0114-035) UCSFi001-A-40 (AICS-094-024) UCSFi001-A-7 (AICS-0017-065) UCSFi001-A-41 (AICS-0034-062) UCSFi001-A-8 (AICS-0010-055) UCSFi001-A-9 (AICS-0014-006) UCSFi001-A-10 (AICS-0023-020) UCSFi001-A-11 (AICS-0024-080) UCSFi001-A-12 (AICS-0036-006) UCSFi001-A-13 (AICS-0022-037) UCSFi001-A-14 (AICS-0025-001) UCSFi001-A-15 (AICS-0025-044) UCSFi001-A-16 (AICS-0031-035) UCSFi001-A-17 (AICS-0032-019) UCSFi001-A-55 (AICS-0078-079) UCSFi001-A-18 (AICS-0030-022) UCSFi001-A-69 (AICS-0083-005) UCSFi001-A-56 (AICS-0088-083) UCSFi001-A-19 (AICS-0033-115) UCSFi001-A-70 (AICS-0083-124) UCSFi001-A-57 (AICS-0099-020) UCSFi001-A-20 (AICS-0040-013) UCSFi001-A-21 (AICS-0040-035) UCSFi001-A-22 (AICS-0053-016) UCSFi001-A-23 (AICS-0054-091) UCSFi001-A-24 (AICS-0057-050) UCSFi001-A-25 (AICS-0058-067) UCSFi001-A-26 (AICS-0037-172) UCSFi001-A-27 (AICS-0048-039) UCSFi001-A-39 (AICS-089-061) |
| Last update | 16th April 2023 |
| Notes | An induced pluripotent stem cell (iPSC) lines carrying the homozygous P525LG (c.1574 C>T) mutation in the FUS gene via genetic modification of a healthy hiPSC line (WTC11, UCSFi001-A). |
| User feedback | |
Provider |
|
| Generator | LSU Health Sciences Center in Shreveport (LSUHS) |
| Owner | LSU Health Sciences Center in Shreveport (LSUHS) |
| Distributors | |
| Derivation country | United States |
External Databases |
|
| BioSamples | SAMEA112670813 |
| Cellosaurus | CVCL_D0NL |
| Wikidata | Q123033687 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Asian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | N/A |
| Is the medical history available upon request? | No |
| Is clinical information available? | Limited clinical information - EKG |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Yes
Normal 46, XY
Karyotyping method:
G-Banding
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA5843920 |
Ethics
Also have a look at the ethics information for the parental line
UCSFi001-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
|
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The source cell information can be found in the parental cell line
UCSFi001-A.
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Reprogramming method |
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| Vector type | None |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
mRNA
|
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method | Enzyme-free cell dissociation |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
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