FUS-R521G HET 2B10, LSUHSi004-A-72
UCSFi001-A-72
General
Cell Line |
|
| hPSCreg Name | UCSFi001-A-72 |
| Alternative name(s) |
FUS-R521G HET 2B10, LSUHSi004-A-72
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 21st February 2023 |
| Notes | The induced pluripotent stem cell (iPSC) lines carrying the heterozygous R521G (c.1561 C>G) mutation in the FUS gene via genetic modification of a healthy hiPSC line (WTC11, UCSFi001-A). |
| User feedback | |
Provider |
|
| Generator | LSU Health Sciences Center in Shreveport (LSUHS) |
| Owner | LSU Health Sciences Center in Shreveport (LSUHS) |
| Distributors | |
| Derivation country | United States |
External Databases |
|
| BioSamples | SAMEA5843920 |
| CLO | CLO_0104467 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Japanese |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | N/A |
| Is the medical history available upon request? | No |
| Is clinical information available? | Limited clinical information - EKG |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
normal karyotype, 46, XY
Karyotyping method:
G-Banding
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA5843920 |
Ethics
Also have a look at the ethics information for the parental line
UCSFi001-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
|
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The source cell information can be found in the parental cell line
UCSFi001-A.
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Reprogramming method |
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| Vector type | None |
Vector free reprogramming |
|
| Type of used vector free reprogramming factor(s) |
mRNA
|
Other |
|
| Selection criteria for clones | Carrying the heterozygous R521G (c.1561 C>G) mutation in the FUS gene. |
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method | Enzyme-free cell dissociation |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
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