FUS-R521G HET 2B10, LSUHSi004-A-72
UCSFi001-A-72
General
iPSC Line |
|
| hPSCreg name | UCSFi001-A-72 |
| Cite as: | UCSFi001-A-72 (RRID:CVCL_D0NI) |
| Alternative name(s) |
FUS-R521G HET 2B10, LSUHSi004-A-72
|
| iPSC line type | Human induced pluripotent stem cell (hiPSC) |
| Similar iPSC lines |
UCSFi001-A-74 (FUS-P525L HET 2D1) UCSFi001-A-75 (FUS-P525L HOM 2D2) UCSFi001-A-73 (FUS-R521G HOM 1D9) UCSFi001-A (wtc11, Wtc11, WTC, WTC11, GM25256) CBIGi001-A-37 (IPSC0109, FUS-H517Q/AIW002-02) CBIGi001-A-38 (IPSC0110, FUS-KO/AIW002-02) WTSIi018-B-24 (Kolf 2.1 J (FUS R495X WT/SNV)) WTSIi018-B-25 (Kolf 2.1 J (FUS R495X SNV/SNV)) WTSIi018-B-26 (Kolf 2.1 J (FUS R521H WT/SNV)) WTSIi018-B-27 (Kolf 2.1 J (FUS R521H SNV/SNV)) UCSFi001-A-49 (YH653-MUT-1C8-HET, DYT1-HET-1C8) UCSFi001-A-50 (YH653-MUT-H6-HET, DYT1-HET-H6) UCSFi001-A-51 (YH653-MUT-2F2-HOMO, DYT1-HOMO-2F2) UCSFi001-A-89 (AICS-0097-102) UCSFi001-A-90 (AICS-0097-141) UCSFi001-A-91 (AICS-0097-157) UCSFi001-A-94 (AICS-0104-003) UCSFi001-A-95 (AICS-0104-085) UCSFi001-A-99 (AICS-0105-030) UCSFi001-A-A (AICS-0105-031) UCSFi001-A-B (AICS-0105-032) UCSFi001-A-C (AICS-0105-057) UCSFi001-A-G (AICS-0106-104) UCSFi001-A-H (AICS-0106-134) UCSFi001-A-I (AICS-0107-009) UCSFi001-A-64 (P633L RBM20 iPSCs) UCSFi001-A-J (AICS-0107-017) UCSFi001-A-M (AICS-0109-056) UCSFi001-A-O (AICS-0109-093) UCSFi001-A-82 (WTC11-SLC12A3) UCSFi001-A-1T (PRKD1-G592R-het) UCSFi001-A-1U (PRKD1-G592R-hom) UCSFi001-A-63 (AICS-0082-001) UCSFi001-A-40 (AICS-094-024) UCSFi001-A-41 (AICS-0034-062) UCSFi001-A-55 (AICS-0078-079) UCSFi001-A-92 (AICS-0097-113) UCSFi001-A-56 (AICS-0088-083) UCSFi001-A-93 (AICS-0097-174) UCSFi001-A-57 (AICS-0099-020) UCSFi001-A-96 (AICS-0104-004) UCSFi001-A-97 (AICS-0104-006) UCSFi001-A-98 (AICS-0105-022) UCSFi001-A-D (AICS-0105-089) UCSFi001-A-E (AICS-0106-040) UCSFi001-A-F (AICS-0106-088) UCSFi001-A-1M (iN-SRCAP-R2444-3) UCSFi001-A-79 (MSE2336A, WTC11-AAVS1-GCamp6s C1) UCSFi001-A-K (AICS-0107-063) |
| Last update | 16th April 2023 |
| Notes | The induced pluripotent stem cell (iPSC) lines carrying the heterozygous R521G (c.1561 C>G) mutation in the FUS gene via genetic modification of a healthy hiPSC line (WTC11, UCSFi001-A). |
| User feedback | |
Provider |
|
| Generator | LSU Health Sciences Center in Shreveport (LSUHS) |
| Owner | LSU Health Sciences Center in Shreveport (LSUHS) |
| Distributors | |
| Derivation country | United States |
External iPSC Databases |
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| BioSamples | SAMEA5843920 |
| Cellosaurus | CVCL_D0NI |
| Wikidata | Q123033684 |
General iPSC Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
iPSC Genetic Modification
| Disease/phenotype related modifications |
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Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Asian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Family history | N/A |
| Is the medical history available upon request? | No |
| Is clinical information available? | Limited clinical information - EKG |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
normal karyotype, 46, XY
Karyotyping method:
G-Banding
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA5843920 |
Ethics
Also have a look at the ethics information for the parental line
UCSFi001-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
iPSC Derivation
General |
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The source cell information can be found in the parental cell line
UCSFi001-A.
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Reprogramming method |
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| Vector type | None |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
mRNA
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Other |
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| Selection criteria for clones | Carrying the heterozygous R521G (c.1561 C>G) mutation in the FUS gene. |
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
No |
iPSC Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method | Enzyme-free cell dissociation |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line? | Yes |
iPSC Characterisation
Analysis of undifferentiated iPSCs
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (iPSC Line) |
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| Has the iPSC line karyotype been analysed? |
Yes
|
Other Genotyping (iPSC Line) |
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