UCSFi001-A-72

FUS-R521G HET 2B10, LSUHSi004-A-72

General

Cell Line

hPSCreg name UCSFi001-A-72
Cite as:
UCSFi001-A-72 (RRID:CVCL_D0NI)
Alternative name(s)
FUS-R521G HET 2B10, LSUHSi004-A-72
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 16th April 2023
Notes The induced pluripotent stem cell (iPSC) lines carrying the heterozygous R521G (c.1561 C>G) mutation in the FUS gene via genetic modification of a healthy hiPSC line (WTC11, UCSFi001-A).
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Provider

Generator LSU Health Sciences Center in Shreveport (LSUHS)
Owner LSU Health Sciences Center in Shreveport (LSUHS)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA5843920
Cellosaurus CVCL_D0NI
Wikidata Q123033684

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Japanese

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history N/A
Is the medical history available upon request? No
Is clinical information available? Limited clinical information - EKG

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
normal karyotype, 46, XY
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA5843920

Ethics

Also have a look at the ethics information for the parental line UCSFi001-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line UCSFi001-A.

Reprogramming method

Vector type None

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA

Other

Selection criteria for clones Carrying the heterozygous R521G (c.1561 C>G) mutation in the FUS gene.
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
SSEA-4
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal 46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • Amyotrophic Lateral Sclerosis
  • ALS
  • Lou Gehrig Disease
  • Amyotrophic lateral sclerosis
show more synonyms
Genetic modifications
FUS (target)
Isogenic modification
16p11.2
c.1561 C>G
R521G
Heterozygous
Mutated