UCSFi001-A-49

YH653-MUT-1C8-HET, DYT1-HET-1C8

General#

Cell Line

hPSCreg Name UCSFi001-A-49
Alternative name(s)
YH653-MUT-1C8-HET, DYT1-HET-1C8
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 7th May 2021
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Provider

Generator University of Louisiana at Lafayette (ULL)

External Databases

BioSamples SAMEA8868384
CLO CLO_0103321

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Asian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history N/A
Is the medical history available upon request? No
Is clinical information available? Limited clinical information - EKG

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
normal karyotype, 46, XY
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA5843920

Ethics#

Also have a look at the ethics information for the parental line UCSFi001-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line UCSFi001-A.

Reprogramming method

Vector type None

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

No characterisation data could be found for this subclone. Please open parental cell line UCSFi001-A

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal 46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Autosomal Dominant Torsion Dystonia 1
Synonyms
  • Autosomal Dominant Torsion Dystonia 1
  • DYT1
Genetic modifications
TOR1A (target)
Gene knock-out
9q34.11
CRISPR-associated (CRISPR/Cas) System