UCSFi001-A-79

MSE2336A, WTC11-AAVS1-GCamp6s C1

General

Cell Line

hPSCreg name UCSFi001-A-79
Cite as:
UCSFi001-A-79
Alternative name(s)
MSE2336A, WTC11-AAVS1-GCamp6s C1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 24th May 2024
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Provider

Generator Icahn School of Medicine at Mount Sinai (ISMMS)
Owner Icahn School of Medicine at Mount Sinai (ISMMS)
Derivation country United States

External Databases

BioSamples SAMEA115658882

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Asian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history N/A
Is the medical history available upon request? No
Is clinical information available? Limited clinical information - EKG

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA5843920

Ethics

Also have a look at the ethics information for the parental line UCSFi001-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

The source cell information can be found in the parental cell line UCSFi001-A.
Passage number reprogrammed P36

Reprogramming method

Vector type Non-integrating

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 0 %
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line UCSFi001-A .

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
Transgene expression
AAVS1 Locus
The UCOE-CAG-GCaMP6 transgene was inserted in the AAVS1 locus
Homologous Recombination