UCSFi001-A-73

FUS-R521G HOM 1D9

The cell line is not validated yet.

General

Cell Line

hPSCreg Name
UCSFi001-A-73
Alternative name(s)
FUS-R521G HOM 1D9
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 21st February 2023
Notes The induced pluripotent stem cell (iPSC) lines carrying the homozygous R521G (c.1561 C>G) mutation in the FUS gene via genetic modification of a healthy hiPSC line (WTC11, UCSFi001-A).
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Provider

Generator LSU Health Sciences Center in Shreveport (LSUHS)
Owner LSU Health Sciences Center in Shreveport (LSUHS)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA112670806
CLO CLO_0104468

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Japanese

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history N/A
Is the medical history available upon request? No
Is clinical information available? Limited clinical information - EKG

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Normal 46, XY
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA5843920

Ethics

Also have a look at the ethics information for the parental line UCSFi001-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line UCSFi001-A.

Reprogramming method

Vector type None

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA

Other

Selection criteria for clones Carrying the homozygous and homozygous R521G (c.1561 C>G) mutation in the FUS gene
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Amyotrophic Lateral Sclerosis
Synonyms
  • Amyotrophic Lateral Sclerosis
  • ALS
  • Lou Gehrig Disease
Genetic modifications
Isogenic modification
16p11.2
c.1561 C>G
R521G
Homozygous
Mutated