BIONi010-C +dox inducible NGN2-GFP

General

Cell Line

hPSCreg name BIONi010-C-15
Cite as:
BIONi010-C-15 (RRID:CVCL_RM87)
Alternative name(s)
BIONi010-C +dox inducible NGN2-GFP
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
BIONi010-C-13
(BIONi010-C + NGN2 #I7-26)
BIONi010-C
(BIONi010-C, K3P53)
BIONi010-C-3
(BIONi010-C ApoE KO #KO30 P30)
BIONi010-C-25
(BIONi010-C heterozygous TREM2 KO)
BIONi010-C-17
(BIONi010-C TREM2 KO)
BIONi010-C-2
(BIONi010-C ApoE E3/E3 #H8 P32)
BIONi010-C-6
(BIONi010-C ApoE E2/E2)
BIONi010-C-7
(BIONi010-C Trem2 R47H)
BIONi010-C-8
(BIONi010-C Trem2 T66M, #Y5-80)
BIONi010-C-9
(BIONi010-C CD33 KO)
BIONi010-C-70
(BIONi010-C with an APOE 2/2 genotype with an additional, homozygous christchurch mutation)
BIONi010-C-71
(BIONi010-C with an APOE 3/3 genotype with an additional, homozygous christchurch mutation)
BIONi010-C-4
(BIONi010-C ApoE E4/E4 #B44 P27)
BIONi010-C-5
(BIONi010-C CD33 E2del #N14 P26)
BIONi010-C-51
(BIONi010-C TNNI3-mCherry reporter)
BIONi010-C-18
(BIONi010-C TBK1 KO)
BIONi010-C-19
(BIONi010-C IKBKE KO)
BIONi010-C-10
(HNF1AP291fsinsC +/- 54-5)
BIONi010-C-11
(HNF1AP291fsinsC -/- 66-1)
BIONi010-C-12
(HNF4ApR309C -/- 2-4)
BIONi010-C-52
(BIONi010-C with an APOE 2/2 genotype (with two functional alleles in contrast to BIONi010-C-6))
BIONi010-C-53
(BIONi010-C with an APOE 3/3 genotype (with two functional alleles in contrast to BIONi010-C-2))
BIONi010-C-55
(BIONi010-C TNNI3-mCherry/TNNI1-EGFP dual reporter cl. 74)
BIONi010-C-24
(BIONi010-C Dox a-syn)
BIONi010-C-43
(BIONi010-C + aSNCA-wt AAVS1)
BIONi010-C-44
(BIONi010-C + aSNCA-A53T AAVS1)
BIONi010-C-64
(BIONi010-C-T2A-Nanoluciferase reporter cl. 29)
BIONi010-C-49
(BIONi010-C + synapsin-m2rtTA + SNCA-wt)
BIONi010-C-50
(BIONi010-C + synapsin-m2rtTA + SNCA-A53T)
BIONi010-C-56
(BIONi010-C-A713T-C25)
BIONi010-C-57
(BIONi010-C-A713T-C42)
BIONi010-C-58
(BIONi010-C-A713T-C1)
BIONi010-C-54
(BIONi010-C with an APOE 4/4 genotype (with two functional alleles in contrast to BIONi010-C-4))
BIONi010-C-59
(BIONi010-C-A713T-C33)
BIONi010-C-60
(BIONi010-C-R589C-C7)
BIONi010-C-61
(BIONi010-C-R589C-C16)
BIONi010-C-62
(BIONi010-C-R589C-C5)
BIONi010-C-63
(BIONi010-C-R589C-C9)
BIONi010-C-48
(BIONi010-C hMDR1)
BIONi010-C-45
(BIONi010-C iCRE AAVS1 GBA1 LoxP EX5-6)
BIONi010-C-41
(BIONi010-C + iNGN2 Two-plasmid system/CRISPR-2)
BIONi010-C-65
(BiONI010-C-O16)
BIONi010-C-66
(BIONi010-C-N7)
BIONi010-C-42
(BIONi010-C + iCRE AAVS1)
BIONi010-A
(K1P53)
BIONi010-B
(K2P53, BIONi010-B)
Last update 7th March 2023
Notes This line contains an NGN2-GFP fusion gene, which is integrated into the AAVS1 locus and inducible by doxycycline. It is isogenic to the line BIONi010-C, which has already been characterized. For stem cell marker expression and differentiation in all three germ layers, please refer to BIONi010-C. No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23
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Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA104008229
Cellosaurus CVCL_RM87
Wikidata Q54796759

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Black or African-American

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

Ethics

Also have a look at the ethics information for the parental line BIONi010-C .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line BIONi010-C.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 18 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Morphology pictures

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 27
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
NEUROG2 (target)
Gene knock-in
AAVS1 locus
GFP
19q13
DOX inducible NGN2-GFP expression triggers transdifferentiation of iPS cells to GFP positive neurons
TALEN