BIONi010-C-15

BIONi010-C +dox inducible NGN2-GFP

General#

Cell Line

hPSCreg Name BIONi010-C-15
Alternative name(s)
BIONi010-C +dox inducible NGN2-GFP
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th November 2018
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA104008229
EBiSC BIONi010-C-15
ECACC 66540570
Cellosaurus CVCL_RM87

General Information

Notes This line contains an NGN2-GFP fusion gene, which is integrated into the AAVS1 locus and inducible by doxycycline. It is isogenic to the line BIONi010-C, which has already been characterized. For stem cell marker expression and differentiation in all three germ layers, please refer to BIONi010-C. No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23
Is the cell line available in principle? Available, without restrictions
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

The source cell information can be found in the parental cell line BIONi010-C.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 18 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Morphology pictures

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
Transgene expression
AAVS1 locus
Enforced NGN2-GFP expression triggers transdifferentiation of iPS cells to GFP positive neurons
TALEN