BIONi010-C-50

BIONi010-C + synapsin-m2rtTA + SNCA-A53T

General

Cell Line

hPSCreg Name
BIONi010-C-50
Alternative name(s)
BIONi010-C + synapsin-m2rtTA + SNCA-A53T
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 16th December 2022
Notes This line contains the tetracycline transactivator m2rtTA under a human synapsin promoter on one allele of the AAVS1 locus and a cDNA of A53T SNCA on the other allele. When differentiated to neurons, the m2rtTA will be expressed. DOX needs to be added to have SNCA-A53T expression. SNCA-A53T should then only be expressed in neurons.
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Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA7111003

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Black or African-American

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

Ethics

Also have a look at the ethics information for the parental line BIONi010-C .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line BIONi010-C.
Passage number reprogrammed 27

Reprogramming method

Vector type Non-integrating
Vector Episomal
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line BIONi010-C .

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
Gene knock-in
AAVS1
This line contains the tetracycline transactivator m2rtTA under a human synapsin promoter on one allele of the AAVS1 locus and a cDNA of A53T SNCA on the other allele. When differentiated to neurons, the m2rtTA will be expressed. DOX needs to be added to have SNCA-A53T expression. SNCA-A53T should then only be expressed in neurons.
CRISPR-associated (CRISPR/Cas) System