BIONi010-C + synapsin-m2rtTA + SNCA-wt
BIONi010-C-49
General
Cell Line |
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| hPSCreg Name | BIONi010-C-49 |
| Alternative name(s) |
BIONi010-C + synapsin-m2rtTA + SNCA-wt
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 16th December 2022 |
| Notes | This line contains the tetracycline transactivator m2rtTA under a human synapsin promoter on one allele of the AAVS1 locus and a cDNA of WT SNCA on the other allele. When differentiated to neurons, the m2rtTA will be expressed. DOX needs to be added to have SNCA-A53T expression. SNCAT should then only be expressed in neurons. |
| User feedback | |
Provider |
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| Generator |
Bioneer (BION)
Contact:
Bioneer (BION) |
| Owner | Bioneer (BION) |
| Distributors | |
| Derivation country | Denmark |
External Databases |
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| BioSamples | SAMEA7111002 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Black or African-American |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA3105780 |
Ethics
Also have a look at the ethics information for the parental line
BIONi010-C
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| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
BIONi010-C.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Methods used |
Immunostaining
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
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Characterisation
No characterisation data could be found for this subclone. Please open parental cell line BIONi010-C .Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
Genetic Modification
| Genetic modifications not related to a disease |
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