BIONi010-C-64

BIONi010-C-T2A-Nanoluciferase reporter cl. 29

General#

Cell Line

hPSCreg Name
BIONi010-C-64
Alternative name(s)
BIONi010-C-T2A-Nanoluciferase reporter cl. 29
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 29th October 2021
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Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA10534760
CLO CLO_0103569

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Black or African-American

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

Ethics#

Also have a look at the ethics information for the parental line BIONi010-C .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? GeneART

hIPSC Derivation#

General

The source cell information can be found in the parental cell line BIONi010-C.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

No characterisation data could be found for this subclone. Please open parental cell line BIONi010-C

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY, normal male karyotype
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
CYP3A4 (target)
Gene knock-in
7q22.1
Nanoluciferase
A T2A-Nanoluciferase transgene was inserted in the wt line BIONi010-C immedeately upstream of the STOP codon of one of the alleles of the CYP3A4 gene using CRISPR/Cas9 resulting in a CYP3A4-T2A-Nanoluciferase reporter
CRISPR-associated (CRISPR/Cas) System
CYP3A4-T2A-Nanoluciferase MAP.jpg
Overview of insertion of T2A-Nanoluciferase
CYP3A4-T2A-Nanoluciferase Ex5 T2A.jpg
Sequence validation of T2A-Nanoluciferase inserted before the STOP codon of CYP3A4
CYP3A4-T2A-Nanoluciferase Ex5 Nanoluc.jpg
Sequence validation of T2A-Nanoluciferase 3' end before the STOP codon of CYP3A4