BIONi010-C-57

BIONi010-C-A713T-C42

General

Cell Line

hPSCreg Name
BIONi010-C-57
Alternative name(s)
BIONi010-C-A713T-C42
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 7th March 2023
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Provider

Generator University of Copenhagen (KU)
Owner University of Copenhagen (KU)
Derivation country Denmark

External Databases

BioSamples SAMEA112445976
CLO CLO_0104220

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Black or African-American

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

Ethics

Also have a look at the ethics information for the parental line BIONi010-C .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

The source cell information can be found in the parental cell line BIONi010-C.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
EDTA
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Unknown
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
SSEA-1
Yes
SSEA-3
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
SMA
Yes
Neuron
Ont Id: CL_0000540
In vitro spontaneous differentiation
Marker Expressed
TUJ1
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 25
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
epilepsy
Synonyms
  • epilepsy
  • Epileptic attack, NOS
  • Epilepsy, unspecified, with intractable epilepsy
  • Generalized seizure (finding)
  • Epileptic seizures
  • Other forms of epilepsy (disorder)
  • Epileptic seizures, NOS
  • [X]Other epilepsy (disorder)
  • Seizure disorder (disorder)
  • Generalized fit
  • Epilepsy (disorder)
  • Epileptic fits
  • Seizure disorder
  • Other forms of epilepsy
  • Epilectic attack, NOS
  • Other forms of epilepsy, with intractable epilepsy
  • Epileptic fits, NOS
  • Epileptic disorder
  • Epilepsy, NOS
  • EPILEP NEC W/O INTR EPIL
  • EPILEPSY NOS W INTR EPIL
  • Epileptic Seizure
  • Epileptic attack
  • Epileptic seizure (finding)
  • seizure disorder
  • [X]Other epilepsy
  • EP - Epilepsy
  • EPILEPSY NEC W INTR EPIL
  • Other forms of epilepsy NOS
  • Other forms of epilepsy and recurrent seizures
  • Epileptic convulsions, NOS
  • Epileptic disorder, NOS
  • Epilepsy, unspecified, without mention of intractable epilepsy
  • Epilepsy, unspecified
  • Epilepsy and recurrent seizures
  • Epilepsy NOS (disorder)
  • EPILEP NOS W/O INTR EPIL
  • Epileptic fit
  • Epilepsy NOS
  • Other forms of epilepsy, without mention of intractable epilepsy
  • Epileptic
  • Generalized seizure
  • Generalized convulsion
  • Generalised seizure
  • Generalised convulsion
  • Other forms of epilepsy NOS (disorder)
  • EF - Epileptic fit
  • Generalised fit
  • Epileptic convulsions
show more synonyms
Genetic modifications
CACNA1A (target)
Variant
Chr19 p13.13
c. 2139G>A
p. Ala713Thr
Homozygous
Genetic modifications not related to a disease
Isogenic modification