JM09 GC
KUi012-A-1
General
Cell Line |
|
| hPSCreg name | KUi012-A-1 |
| Cite as: | KUi012-A-1 |
| Alternative name(s) |
JM09 GC
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
KUi012-A (JM09) KUi013-A-1 (SK GC) BIONi010-C-58 (BIONi010-C-A713T-C1) BIONi010-C-59 (BIONi010-C-A713T-C33) BIONi010-C-60 (BIONi010-C-R589C-C7) BIONi010-C-61 (BIONi010-C-R589C-C16) BIONi010-C-62 (BIONi010-C-R589C-C5) BIONi010-C-63 (BIONi010-C-R589C-C9) BIONi010-C-56 (BIONi010-C-A713T-C25) BIONi010-C-57 (BIONi010-C-A713T-C42) UCSFi001-A-49 (YH653-MUT-1C8-HET, DYT1-HET-1C8) UCSFi001-A-50 (YH653-MUT-H6-HET, DYT1-HET-H6) UCSFi001-A-51 (YH653-MUT-2F2-HOMO, DYT1-HOMO-2F2) |
| Last update | 25th October 2023 |
| User feedback | |
Provider |
|
| Generator |
University of Copenhagen (KU)
Contact:
University of Copenhagen (KU) |
| Owner | University of Copenhagen (KU) |
| Derivation country | Denmark |
External Databases |
|
| BioSamples | SAMEA114438234 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Cell line can only be used in: Epilepsy
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
It requires an MTA agreement |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA114437882 |
Ethics
Also have a look at the ethics information for the parental line
KUi012-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
KUi012-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Yes |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
EDTA
|
| Medium |
Essential 8™
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| SOX2 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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