General#

Cell Line
hPSCreg Name A standardised name that was automatically generated based on the cell line information. see Naming Tool	BJTTHi001-A-2
Cell line type	Human induced pluripotent stem cell (hiPSC)
Last update	6th March 2021
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Provider
Generator	Beijing Tiantan Hospital (BJTTH)
External Databases
BioSamples	SAMEA8359796
CLO	CLO_0103206
Cellosaurus	CVCL_A8HC
Wikidata	Q107113467
General Information
* Is the cell line readily obtainable for third parties?	No
Subclone of	BJTTHi001-A

Donor Information#

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
External Databases (Donor)
BioSamples	SAMEA8172002

Ethics#

Also have a look at the ethics information for the parental line BJTTHi001-A .

For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General
The source cell information can be found in the parental cell line BJTTHi001-A.
Reprogramming method
Vector type	None
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions#

Surface coating	Matrigel/Geltrex
Feeder cells	No
Medium	Other medium: Base medium: Nuwacell ncTarget hPSC Medium Main protein source: Serum concentration: %

Characterisation#

No characterisation data could be found for this subclone. Please open parental cell line BJTTHi001-A .

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46,XY 3H.tif Karyotyping method: G-Banding
Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications

Dravet syndrome Dravet syndrome in the OLS Genetic modifications SCN1B (target) Type of modification Gene knock-out Chromosome location 19q13.11 Delivery method CRISPR-associated (CRISPR/Cas) System epilepsy epilepsy in the OLS Synonyms epilepsy Epileptic attack, NOS Epilepsy, unspecified, with intractable epilepsy Generalized seizure (finding) Epileptic seizures Other forms of epilepsy (disorder) Epileptic seizures, NOS [X]Other epilepsy (disorder) Seizure disorder (disorder) Generalized fit Epilepsy (disorder) Epileptic fits Seizure disorder Other forms of epilepsy Epilectic attack, NOS Other forms of epilepsy, with intractable epilepsy Epileptic fits, NOS Epileptic disorder Epilepsy, NOS EPILEP NEC W/O INTR EPIL EPILEPSY NOS W INTR EPIL Epileptic Seizure Epileptic attack Epileptic seizure (finding) seizure disorder [X]Other epilepsy EP - Epilepsy EPILEPSY NEC W INTR EPIL Other forms of epilepsy NOS Other forms of epilepsy and recurrent seizures Epileptic convulsions, NOS Epileptic disorder, NOS Epilepsy, unspecified, without mention of intractable epilepsy Epilepsy, unspecified Epilepsy and recurrent seizures Epilepsy NOS (disorder) EPILEP NOS W/O INTR EPIL Epileptic fit Epilepsy NOS Other forms of epilepsy, without mention of intractable epilepsy Epileptic Generalized seizure Generalized convulsion Generalised seizure Generalised convulsion Other forms of epilepsy NOS (disorder) EF - Epileptic fit Generalised fit Epileptic convulsions show more synonyms show less synonyms Genetic modifications SCN1B (target) Type of modification Gene knock-out Chromosome location 19q13.11 Delivery method CRISPR-associated (CRISPR/Cas) System