General

Cell Line
hPSCreg name	BJTTHi001-A-3
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	BJTTHi001-A-3 (RRID:CVCL_C1SQ)
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	BJTTHi001-A BJTTHi001-A-1 BJTTHi001-A-2 BJTTHi001-A-4 BJTTHi001-A-5 BIONi010-C-58 (BIONi010-C-A713T-C1) BIONi010-C-59 (BIONi010-C-A713T-C33) BIONi010-C-60 (BIONi010-C-R589C-C7) BIONi010-C-61 (BIONi010-C-R589C-C16) BIONi010-C-62 (BIONi010-C-R589C-C5) BIONi010-C-63 (BIONi010-C-R589C-C9) KUi012-A-1 (JM09 GC) BIONi010-C-56 (BIONi010-C-A713T-C25) BIONi010-C-57 (BIONi010-C-A713T-C42) UCSFi001-A-49 (YH653-MUT-1C8-HET, DYT1-HET-1C8) UCSFi001-A-50 (YH653-MUT-H6-HET, DYT1-HET-H6) UCSFi001-A-51 (YH653-MUT-2F2-HOMO, DYT1-HOMO-2F2) WAe001-A-53
Last update	25th May 2022
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Provider
Generator	Beijing Tiantan Hospital (BJTTH)
External Databases
BioSamples	SAMEA14449092
Cellosaurus	CVCL_C1SQ
Wikidata	Q114310754
General Information
* Is the cell line readily obtainable for third parties?	No
Subclone of	BJTTHi001-A

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
Karyotyping (Donor)
Has the donor karyotype been analysed?	Unknown
External Databases (Donor)
BioSamples	SAMEA8172002

Ethics

Also have a look at the ethics information for the parental line BJTTHi001-A .

For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line BJTTHi001-A.
Reprogramming method
Vector type	None
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Passage method	Enzyme-free cell dissociation EDTA
CO2 Concentration	5 %
Medium	Other medium: Base medium: ncTarget hPSC Medium Main protein source: Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line BJTTHi001-A .

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46,XY KCNA2.pdf
Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications

Epilepsy Synonyms epilepsy Epileptic attack, NOS Epilepsy, unspecified, with intractable epilepsy Generalized seizure (finding) Epileptic seizures Other forms of epilepsy (disorder) Epileptic seizures, NOS [X]Other epilepsy (disorder) Seizure disorder (disorder) Generalized fit Epilepsy (disorder) Epileptic fits Seizure disorder Other forms of epilepsy Epilectic attack, NOS Other forms of epilepsy, with intractable epilepsy Epileptic fits, NOS Epileptic disorder Epilepsy, NOS EPILEP NEC W/O INTR EPIL EPILEPSY NOS W INTR EPIL Epileptic Seizure Epileptic attack Epileptic seizure (finding) seizure disorder [X]Other epilepsy EP - Epilepsy EPILEPSY NEC W INTR EPIL Other forms of epilepsy NOS Other forms of epilepsy and recurrent seizures Epileptic convulsions, NOS Epileptic disorder, NOS Epilepsy, unspecified, without mention of intractable epilepsy Epilepsy, unspecified Epilepsy and recurrent seizures Epilepsy NOS (disorder) EPILEP NOS W/O INTR EPIL Epileptic fit Epilepsy NOS Other forms of epilepsy, without mention of intractable epilepsy Epileptic Generalized seizure Generalized convulsion Generalised seizure Generalised convulsion Other forms of epilepsy NOS (disorder) EF - Epileptic fit Generalised fit Epileptic convulsions show more synonyms show less synonyms Genetic modifications KCNA2 (target) Type of modification Gene knock-out Delivery method CRISPR-associated (CRISPR/Cas) System