SK GC
KUi013-A-1
General
Cell Line |
|
| hPSCreg name | KUi013-A-1 |
| Cite as: | KUi013-A-1 (RRID:CVCL_D6PE) |
| Alternative name(s) |
SK GC
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
KUi013-A (SK) KUi012-A-1 (JM09 GC) SIGi001-A-3 (iPSC0028 MAPT P301L C4) SIGi001-A-4 (iPSC0028 MAPT P301L D4) SIGi001-A-5 (iPSC0028 MAPT P301S 1C9C4) SIGi001-A-6 (iPSC0028 MAPT P301S 1B9C9) SIGi001-A-7 (iPSC0028 MAPT P301S 2G2B7) SIGi001-A-8 (iPSC0028 MAPT P301S+Ex10+16/Clone 7D11A7) SIGi001-A-9 (iPSC0028 MAPT P301S+Ex10+16/Clone 7H6A1) SIGi001-A-10 (iPSC0028 MAPT P301S+Ex10+16/Clone 7G4A8) SIGi001-A-11 (iPSC0028 MAPT P301S+Ex10+16/Clone 7C6A4, SAMEA4451118) SIGi001-A-12 (iPSC0028 - BiAllelic MAPT_Ex10+16T/Clone 1F5-D12, SAMEA104237570) SIGi001-A-16 (SIGi001-A-9 Bi-Allelic MAPT HA-tag) |
| Last update | 25th October 2023 |
| User feedback | |
Provider |
|
| Generator |
University of Copenhagen (KU)
Contact:
University of Copenhagen (KU) |
| Owner | University of Copenhagen (KU) |
| Derivation country | Denmark |
External Databases |
|
| BioSamples | SAMEA114440046 |
| Cellosaurus | CVCL_D6PE, CVCL_D6PG |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Cell line can only be used in: Epilepsy
Additional restrictions:
It requires an MTA agreement. |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA114440047 |
Ethics
Also have a look at the ethics information for the parental line
KUi013-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
KUi013-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Yes |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
EDTA
|
| Medium |
Essential 8™
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
i have uploaded the SNP analysis for the detecting the chromosomal abnormalities. No abnormalities detected.
Karyotyping method:
SNP analysis
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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