SIGi001-A-9 Bi-Allelic MAPT HA-tag
SIGi001-A-16
General#
Cell Line |
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hPSCreg Name | SIGi001-A-16 |
Alternative name(s) |
SIGi001-A-9 Bi-Allelic MAPT HA-tag
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 20th April 2021 |
Notes | SIGi001-A-9 (iPSC0028 MAPT P301S+Ex10+16/Clone 7H6A1) having a HA-tag at the C-terminal end of MAPT, Bi-Allelic |
User feedback | |
Provider |
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Generator |
Bioneer (BION)
Contact:
Bioneer (BION) |
Owner | Bioneer (BION) |
Distributors | |
External Databases |
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BioSamples | SAMEA6080815 |
CLO | CLO_0103295 |
Cellosaurus | CVCL_A7GT |
Wikidata | Q107116926 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: not allowed
Commercial: not allowed
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Subclone of |
Donor Information#
General Donor Information |
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Sex | female |
Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Disease associated phenotypes |
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Family history | no |
Is the medical history available upon request? | no |
Is clinical information available? | no |
Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
|
BioSamples | SAMEA4447434 |
Ethics#
Also have a look at the ethics information for the parental line
SIGi001-A
.
Is there an MTA available for the cell line? | Yes |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Bioneer |
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation#
General |
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The source cell information can be found in the parental cell line
SIGi001-A.
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Passage number reprogrammed | +15 |
Reprogramming method |
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Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
Unknown |
Reprogramming vectors silenced? |
Unknown |
Vector free reprogramming |
|
Other |
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Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzymatically
Accutase
|
O2 Concentration | 5 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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SOX2 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-81 |
Yes |
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SSEA-1 |
No |
Morphology pictures
Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46,XX
Passage number: +15
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
Genetic Modification#
Disease/phenotype related modifications |
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