iPSC0028 MAPT P301S+Ex10+16/Clone 7G4A8
SIGi001-A-10
General#
Cell Line |
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hPSCreg Name | SIGi001-A-10 |
Alternative name(s) |
iPSC0028 MAPT P301S+Ex10+16/Clone 7G4A8
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 7th February 2019 |
User feedback | |
Provider |
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Generator | Sigma-Aldrich (SIG) |
Distributors | |
External Databases |
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BioSamples | SAMEA4451117 |
Cellosaurus | CVCL_II92 |
ECACC | 66540358 |
EBiSC | SIGi001-A-10 |
General Information |
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Projects | |
Is the cell line available in principle? | Available with restrictions: Available for clinical and commercial research |
Subclone of |
Donor Information#
General Donor Information |
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Sex | female |
Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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External Databases (Donor) |
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BioSamples | SAMEA4447434 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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The source cell information can be found in the parental cell line
SIGi001-A.
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Reprogramming method |
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Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
Unkown |
Reprogramming vectors silenced? | |
Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
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O2 Concentration | 21 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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TRA 1-60 |
Yes |
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SSEA-4 |
Yes |
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SSEA-1 |
No |
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Differentiation Potency
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
No autosomal or sex chromosome abnormalities detected
Karyotyping method:
Karyolite BoBs
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Other Genotyping (Cell Line) |
Genetic Modification#
Disease/phenotype related modifications |
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