hPSCreg
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SIGi001-A-10

Registration Summary:

iPSC0028 MAPT P301S+Ex10+16/Clone 7G4A8

SIGi001-A-10

General#

Cell Line
hPSCreg Name SIGi001-A-10
Alternative name(s)
iPSC0028 MAPT P301S+Ex10+16/Clone 7G4A8
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 23rd October 2017
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Provider
Generator Sigma-Aldrich (SIG)
Distributors

External Databases
BioSamples SAMEA4451117
Cellosaurus CVCL_II92
ECACC 66540358
EBiSC SIGi001-A-10

General Information
Is the cell line available in principle? Available with restrictions: Available for clinical and commercial research
Subclone of

Donor Information#

General Donor Information
Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.

External Databases (Donor)
BioSamples SAMEA4447434

Ethics#

We are currently revising this section.

hIPSC Derivation#

General
The source cell information can be found in the parental cell line SIGi001-A.

Reprogramming method
Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Unkown
Reprogramming vectors silenced?

Vector free reprogramming

Other
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
SSEA-4
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
GATA6
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes

Microbiology / Virus Screening
HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
No autosomal or sex chromosome abnormalities detected
Karyotyping method: Karyolite BoBs

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Progressive supranuclear palsy
Progressive supranuclear palsy in the OLS
There are three disease associations for the edited subclone: PSP (progressive supranuclear palsy) /CBD (Corticobasal degeneration) / FTDP-17 (Frontotemporal dementia and parkinsonism linked to chromosome 17) - like symptoms
Synonyms
  • PSP syndrome
Genetic modifications
MAPT (target)
Isogenic modification
17q21.31
NM_016834.4 : c.[ c.727 >T; c.[741+16C>T]+[741+16C>T]]
Homozygous
P301S+IVS10+16 C>T
Mutated
OBSOLETE: Corticobasal degeneration
OBSOLETE: Corticobasal degeneration in the OLS
There are three disease associations for the edited subclone: PSP (progressive supranuclear palsy) /CBD (Corticobasal degeneration) / FTDP-17 (Frontotemporal dementia and parkinsonism linked to chromosome 17) - like symptoms
Genetic modifications
MAPT (target)
Isogenic modification
17q21.31
NM_016834.4 : c.[ c.727 >T; c.[741+16C>T]+[741+16C>T]]
Homozygous
P301S+IVS10+16 C>T
Mutated
FTDP-17 (Frontotemporal dementia and parkinsonism linked to chromosome 17) has no ontology id.
Genetic modifications
MAPT (target)
Isogenic modification
17q21.31
NM_016834.4 : c.[ c.727 >T; c.[741+16C>T]+[741+16C>T]]
Homozygous
P301S+IVS10+16 C>T
Mutated