SIGi001-A-9 Dox inducible NGN2
SIGi001-A-17
General
Cell Line |
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hPSCreg name | SIGi001-A-17 |
Cite as: | SIGi001-A-17 |
Alternative name(s) |
SIGi001-A-9 Dox inducible NGN2
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
SIGi001-A (IPSC0028 parental, SAMEA4447455) SIGi001-A-1 (iPSC0028 SLC17A7/GFP E3) SIGi001-A-2 (iPSC0028 SLC17A7/GFP C3) SIGi001-A-3 (iPSC0028 MAPT P301L C4) SIGi001-A-4 (iPSC0028 MAPT P301L D4) SIGi001-A-5 (iPSC0028 MAPT P301S 1C9C4) SIGi001-A-6 (iPSC0028 MAPT P301S 1B9C9) SIGi001-A-7 (iPSC0028 MAPT P301S 2G2B7) SIGi001-A-8 (iPSC0028 MAPT P301S+Ex10+16/Clone 7D11A7) SIGi001-A-9 (iPSC0028 MAPT P301S+Ex10+16/Clone 7H6A1) SIGi001-A-10 (iPSC0028 MAPT P301S+Ex10+16/Clone 7G4A8) SIGi001-A-11 (iPSC0028 MAPT P301S+Ex10+16/Clone 7C6A4, SAMEA4451118) SIGi001-A-21 (SIGi001-AAVS1-FRT) SIGi001-A-22 (SIGi001-ICAM1-GFP) SIGi001-A-23 (SIGi001-HC3x) SIGi001-A-24 (SIGi001-HC3x-ICAM1-GFP) SIGi001-A-12 (iPSC0028 - BiAllelic MAPT_Ex10+16T/Clone 1F5-D12, SAMEA104237570) SIGi001-A-13 (iPSC0028 – MonoAllelic MAPT_Ex10+16T/Clone 1D01-11) SIGi001-A-14 (SIGi001-A Bi-Allelic MAPT HA-tag) SIGi001-A-15 (SIGi001-A Dox inducible NGN2) SIGi001-A-16 (SIGi001-A-9 Bi-Allelic MAPT HA-tag) SIGi001-A-18 (SIGi001-A-12 Bi-Allelic MAPT HA-tag #U28-12-P29-5) SIGi001-A-19 (SIGi001-A-12 Dox inducible NGN2) |
Last update | 5th January 2024 |
User feedback | |
Provider |
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Generator | Bioneer (BION) |
Owner | Bioneer (BION) |
Distributors | |
External Databases |
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BioSamples | SAMEA6080816 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Subclone of |
Donor Information
General Donor Information |
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Sex | female |
Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Family history | no |
Is the medical history available upon request? | no |
Is clinical information available? | no |
Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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BioSamples | SAMEA4447434 |
Ethics
Also have a look at the ethics information for the parental line
SIGi001-A
.
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
SIGi001-A.
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Reprogramming method |
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Vector type | Integrating |
Vector | Virus (Retrovirus) |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
No |
Reprogramming vectors silenced? |
Unknown |
Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzymatically
Accutase
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O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
SSEA-1 |
No |
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POU5F1 (OCT-4) |
Yes |
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SSEA-4 |
Yes |
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Differentiation Potency
In vitro directed differentiation
Protocol or reference
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
Genetic Modification
Genetic modifications not related to a disease |
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