SIGi001-A-9 Dox inducible NGN2
SIGi001-A-17
General
Cell Line |
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| hPSCreg Name | SIGi001-A-17 |
| Alternative name(s) |
SIGi001-A-9 Dox inducible NGN2
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | |
| Last update | 2nd October 2022 |
| User feedback | |
Provider |
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| Generator | Bioneer (BION) |
| Owner | Bioneer (BION) |
| Distributors | |
External Databases |
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| BioSamples | SAMEA6080816 |
| EBiSC | SIGi001-A-17 |
| ECACC | 66541216 |
| CLO | CLO_0104085 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Family history | no |
| Is the medical history available upon request? | no |
| Is clinical information available? | no |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA4447434 |
Ethics
Also have a look at the ethics information for the parental line
SIGi001-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
SIGi001-A.
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Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Retrovirus) |
| Is the used vector excisable? |
No |
| Absence of reprogramming vector(s)? |
No |
| Reprogramming vectors silenced? |
Unknown |
| Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
Accutase
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Characterisation
No characterisation data could be found for this subclone. Please open parental cell line SIGi001-A .Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
Genetic Modification
| Genetic modifications not related to a disease |
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