SIGi001-A-9 Dox inducible NGN2
SIGi001-A-17
General
Cell Line |
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hPSCreg Name | SIGi001-A-17 |
Alternative name(s) |
SIGi001-A-9 Dox inducible NGN2
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | |
Last update | 2nd October 2022 |
User feedback | |
Provider |
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Generator | Bioneer (BION) |
Owner | Bioneer (BION) |
Distributors | |
External Databases |
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BioSamples | SAMEA6080816 |
EBiSC | SIGi001-A-17 |
ECACC | 66541216 |
CLO | CLO_0104085 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Subclone of |
Donor Information
General Donor Information |
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Sex | female |
Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Family history | no |
Is the medical history available upon request? | no |
Is clinical information available? | no |
Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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BioSamples | SAMEA4447434 |
Ethics
Also have a look at the ethics information for the parental line
SIGi001-A
.
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
SIGi001-A.
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Reprogramming method |
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Vector type | Integrating |
Vector | Virus (Retrovirus) |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
No |
Reprogramming vectors silenced? |
Unknown |
Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzymatically
Accutase
|
O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Characterisation
No characterisation data could be found for this subclone. Please open parental cell line SIGi001-A .Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
Genetic Modification
Genetic modifications not related to a disease |
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