iPSC0028 MAPT P301S 1B9C9
SIGi001-A-6
General#
Cell Line |
|
hPSCreg Name | SIGi001-A-6 |
Alternative name(s) |
iPSC0028 MAPT P301S 1B9C9
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 23rd October 2017 |
User feedback | |
Provider |
|
Generator | Sigma-Aldrich (SIG) |
Owner | Janssen Pharmaceutica (JP) |
Distributors | |
External Databases |
|
BioSamples | SAMEA4447426 |
Cellosaurus | CVCL_EF87 |
ECACC | 66540326 |
EBiSC | SIGi001-A-6 |
General Information |
|
Projects | |
Is the cell line available in principle? | Available with restrictions: Available for clinical and commercial research |
Subclone of |
Donor Information#
General Donor Information |
|
Sex | female |
Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
BioSamples | SAMEA4447434 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
|
The source cell information can be found in the parental cell line
SIGi001-A.
|
|
Reprogramming method |
|
Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
Unkown |
Reprogramming vectors silenced? | |
Vector free reprogramming |
|
Other |
|
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 21 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
|
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
|
||||
SSEA-4 |
Yes |
|
||||
TRA 1-60 |
Yes |
|
||||
SSEA-1 |
No |
|
Differentiation Potency
In vitro spontaneous differentiation
Marker | Expressed |
GATA6 |
Yes |
TBX5 |
Yes |
In vitro spontaneous differentiation
Marker | Expressed |
PAX6 |
Yes |
NeuroD |
Yes |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
Genetic Modification#
Disease/phenotype related modifications |
|
Login to share your feedback, experiences or results with the research community.