iPSC0028 MAPT P301S 1B9C9
SIGi001-A-6
General#
Cell Line |
|
| hPSCreg Name | SIGi001-A-6 |
| Alternative name(s) |
iPSC0028 MAPT P301S 1B9C9
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 23rd October 2017 |
| User feedback | |
Provider |
|
| Generator | Sigma-Aldrich (SIG) |
| Owner | Janssen Pharmaceutica (JP) |
| Distributors | |
External Databases |
|
| BioSamples | SAMEA4447426 |
| Cellosaurus | CVCL_EF87 |
| ECACC | 66540326 |
| EBiSC | SIGi001-A-6 |
General Information |
|
| Is the cell line available in principle? | Available with restrictions: Available for clinical and commercial research |
| Subclone of | |
Donor Information#
General Donor Information |
|
| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA4447434 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
|
|
The source cell information can be found in the parental cell line
SIGi001-A.
|
|
Reprogramming method |
|
| Vector type | Integrating |
| Vector | Virus (Retrovirus) |
| Genes | |
| Is the used vector excisable? |
No |
| Absence of reprogramming vector(s)? |
Unkown |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 21 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|
||||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
||||
| SSEA-1 |
No |
|
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| GATA6 |
Yes |
| TBX5 |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| PAX6 |
Yes |
| NeuroD |
Yes |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
|
Genetic Modification#
| Disease/phenotype related modifications |
|
Login to share your feedback, experiences or results with the research community.