SIGi001-A-9

iPSC0028 MAPT P301S+Ex10+16/Clone 7H6A1

General#

Cell Line

hPSCreg Name SIGi001-A-9
Alternative name(s)
iPSC0028 MAPT P301S+Ex10+16/Clone 7H6A1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 30th July 2018
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Provider

Generator Sigma-Aldrich (SIG)
Owner Janssen Pharmaceutica (JP)
Distributors

External Databases

BioSamples SAMEA4447499
Cellosaurus CVCL_EF90
ECACC 66540327
EBiSC SIGi001-A-9

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for clinical and commercial research
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA4447434

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

The source cell information can be found in the parental cell line SIGi001-A.

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Unkown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
GATA6
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Progressive supranuclear palsy
There are three disease associations for the edited subclone: PSP (progressive supranuclear palsy) /CBD (Corticobasal degeneration) / FTDP-17 (Frontotemporal dementia and parkinsonism linked to chromosome 17) - like symptoms
Synonyms
  • PSP syndrome
Genetic modifications
MAPT (target)
Isogenic modification
17q21.31
NM_016834.4 : c.[ c.[727C>T]+[727C>T]; c.[741+16C>T]+[741+16C>T]]
Homozygous
P301S+IVS10+16 C>T; This clone is homozygous for the IVS10+16 as well as P301S mutation.
Mutated
OBSOLETE: Corticobasal degeneration
Genetic modifications
MAPT (target)
Isogenic modification
17q21.31
NM_016834.4 : c.[ c.[727C>T]+[727C>T]; c.[741+16C>T]+[741+16C>T]]
P301S+IVS10+16 C>T; This clone is homozygous for the IVS10+16 as well as P301S mutation.
Mutated
FTDP-17 (Frontotemporal dementia and parkinsonism linked to chromosome 17) has no ontology id.
Genetic modifications
MAPT (target)
Isogenic modification
17q21.31
NM_016834.4 : c.[ c.[727C>T]+[727C>T]; c.[741+16C>T]+[741+16C>T]]
P301S+IVS10+16 C>T; This clone is homozygous for the IVS10+16 as well as P301S mutation.
Mutated