SIGi001-A-21

SIGi001-AAVS1-FRT

General

Cell Line

hPSCreg Name
SIGi001-A-21
Alternative name(s)
SIGi001-AAVS1-FRT
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 21st July 2022
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator KU Leuven (KUL)
Distributors
Derivation country Belgium

External Databases

BioSamples SAMEA4451096
CLO CLO_0103930

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: allowed
Additional restrictions:

provided license for ZFN and iPSC

Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history no
Is the medical history available upon request? no
Is clinical information available? no

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA4447434

Ethics

Also have a look at the ethics information for the parental line SIGi001-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? ZFN from Sigma

hIPSC Derivation

General

The source cell information can be found in the parental cell line SIGi001-A.

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?
Yes

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™ Flex
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
NANOG
Yes
SOX2
Yes
Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Liver
Ont Id: UBERON_0002107
In vitro directed differentiation
Scorecard
Blood Cell
Ont Id: CL_0000081
In vitro directed differentiation
Neuron
Ont Id: CL_0000540
In vitro directed differentiation

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Array CGH
no genome wide variations

Genetic Modification

Genetic modifications not related to a disease
Transgene expression
FRT-Hyg-TK-FRT cassette in the AAVS1 locus
Zinc finger nuclease