SIGi001-A-25
General
Cell Line |
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hPSCreg name | SIGi001-A-25 |
Cite as: | SIGi001-A-25 |
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
SIGi001-A (IPSC0028 parental, SAMEA4447455) SIGi001-A-1 (iPSC0028 SLC17A7/GFP E3) SIGi001-A-2 (iPSC0028 SLC17A7/GFP C3) SIGi001-A-3 (iPSC0028 MAPT P301L C4) SIGi001-A-4 (iPSC0028 MAPT P301L D4) SIGi001-A-5 (iPSC0028 MAPT P301S 1C9C4) SIGi001-A-6 (iPSC0028 MAPT P301S 1B9C9) SIGi001-A-7 (iPSC0028 MAPT P301S 2G2B7) SIGi001-A-8 (iPSC0028 MAPT P301S+Ex10+16/Clone 7D11A7) SIGi001-A-9 (iPSC0028 MAPT P301S+Ex10+16/Clone 7H6A1) SIGi001-A-10 (iPSC0028 MAPT P301S+Ex10+16/Clone 7G4A8) SIGi001-A-11 (iPSC0028 MAPT P301S+Ex10+16/Clone 7C6A4, SAMEA4451118) SIGi001-A-21 (SIGi001-AAVS1-FRT) SIGi001-A-22 (SIGi001-ICAM1-GFP) SIGi001-A-23 (SIGi001-HC3x) SIGi001-A-24 (SIGi001-HC3x-ICAM1-GFP) SIGi001-A-12 (iPSC0028 - BiAllelic MAPT_Ex10+16T/Clone 1F5-D12, SAMEA104237570) SIGi001-A-13 (iPSC0028 – MonoAllelic MAPT_Ex10+16T/Clone 1D01-11) SIGi001-A-14 (SIGi001-A Bi-Allelic MAPT HA-tag) SIGi001-A-15 (SIGi001-A Dox inducible NGN2) SIGi001-A-16 (SIGi001-A-9 Bi-Allelic MAPT HA-tag) SIGi001-A-17 (SIGi001-A-9 Dox inducible NGN2) SIGi001-A-18 (SIGi001-A-12 Bi-Allelic MAPT HA-tag #U28-12-P29-5) SIGi001-A-19 (SIGi001-A-12 Dox inducible NGN2) |
Last update | 2nd February 2023 |
Notes | an eGFP -linker sequence was introduced 3' from the BIP locus |
User feedback | |
Provider |
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Generator | KU Leuven (KUL) |
Owner | KU Leuven (KUL) |
Distributors | |
External Databases |
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BioSamples | SAMEA112466072 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Commercial use: allowed
Additional restrictions:
restrictions for commercial entities for addgene sequences, CRISPR/Cas and iPSC for which licenses are needed |
Subclone of |
Donor Information
General Donor Information |
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Sex | female |
Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Family history | no |
Is the medical history available upon request? | no |
Is clinical information available? | no |
Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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BioSamples | SAMEA4447434 |
Ethics
Also have a look at the ethics information for the parental line
SIGi001-A
.
Is there an MTA available for the cell line? | Yes |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | addgene and CRISPR/Cas |
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
Constraints for use or distribution | for commercial use, licenses from depositors at addgene and CRIPSR/Cas needed |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
SIGi001-A.
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Reprogramming method |
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Vector type | Integrating |
Vector | Virus (Retrovirus) |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
No |
Reprogramming vectors silenced? |
Yes |
Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions
Surface coating | Laminin |
Feeder cells |
No |
Passage method |
Enzymatically
Accutase
|
O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™ Flex
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
No characterisation data could be found for this subclone. Please open parental cell line SIGi001-A .Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Unknown
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Other Genotyping (Cell Line) |
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Is there genome-wide genotyping or functional data available? |
Yes
Array CGH
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Genetic Modification
Genetic modifications not related to a disease |
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