SIGi001-A-2

iPSC0028 SLC17A7/GFP C3

General#

Cell Line

hPSCreg Name SIGi001-A-2
Alternative name(s)
iPSC0028 SLC17A7/GFP C3
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 23rd October 2017
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Provider

Generator Sigma-Aldrich (SIG)
Distributors

External Databases

BioSamples SAMEA4451116
Cellosaurus CVCL_II91
ECACC 66540357
EBiSC SIGi001-A-2

General Information

Is the cell line available in principle? Available with restrictions: Available for clinical and commercial research
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA4447434

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

The source cell information can be found in the parental cell line SIGi001-A.

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Unkown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
GATA6
Yes
TBX5
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
SERPINA
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
NEUROD
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No autosomal or sex chromosome aneuploidies detected
Karyotyping method: Karyolite BOBs

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
SLC17A7 (target)
Gene knock-in
19q13.33
T2A-GFP cassette
Induced pluripotent stem cell line with insertion of T2A-GFP cassette into SLC17a7 gene introduced by zinc finger nuclease (ZFN) technology.
Zinc finger nuclease