SIGi001-A-14

SIGi001-A Bi-Allelic MAPT HA-tag

General#

Cell Line

hPSCreg Name
SIGi001-A-14
Alternative name(s)
SIGi001-A Bi-Allelic MAPT HA-tag
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 23rd April 2021
Notes SIGi001-A having a HA-tag at the C-terminal end of MAPT, Bi-Allelic
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Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA6080813
ECACC 66541213
CLO CLO_0103293
Cellosaurus CVCL_A7GS

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Frontotemporal Dementia
In the original line SIGi001-A, no disease was diagnosed. In the line SIGi001-A-14, an HA-tag was added in homozygosity
Synonyms
  • Frontotemporal Dementia
Family history no
Is the medical history available upon request? no
Is clinical information available? no

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA4447434

Ethics#

Also have a look at the ethics information for the parental line SIGi001-A .
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Bioneer
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation#

General

The source cell information can be found in the parental cell line SIGi001-A.
Passage number reprogrammed 0

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Unknown
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Stem cell marker and Tri-lineage differentiation by flow cytometry
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Morphology

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Passage number: +15

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
MAPT (target)
Isogenic modification
17
NM_001203252
Homozygous
an HA-tag has been added to the endogenous MAPT gene
an HA-tag has been added to the endogenous MAPT gene