SIGi001-A-11

iPSC0028 MAPT P301S+Ex10+16/Clone 7C6A4, SAMEA4451118

General#

Cell Line

hPSCreg Name SIGi001-A-11
Alternative name(s)
iPSC0028 MAPT P301S+Ex10+16/Clone 7C6A4, SAMEA4451118
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 7th August 2019
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Provider

Generator Sigma-Aldrich (SIG)
Distributors

External Databases

BioSamples SAMEA4451118
Cellosaurus CVCL_LE50
ECACC 66540359
EBiSC SIGi001-A-11

General Information

Notes The parental iPSC line SIGi001-A can be sourced via Sigma as 'iPSC0028'.
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA4447434

Ethics#

Also have a look at the ethics information for the parental line SIGi001-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line SIGi001-A.

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
CXCR4
Yes
GATA6
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
VIM
Yes
MIXL1
Yes
NCAM1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
NeuroD1
Yes
PAX6
Yes
HES5
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 37

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX[17]/47,XX,+12[2]
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755
This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee https://ega-archive.org/dacs/EGAC00001000768

Genetic Modification#

Disease/phenotype related modifications
Progressive supranuclear palsy
There are three disease associations for the edited subclone: PSP (progressive supranuclear palsy) /CBD (Corticobasal degeneration) / FTDP-17 (Frontotemporal dementia and parkinsonism linked to chromosome 17) - like symptoms
Synonyms
  • PSP syndrome
Genetic modifications
MAPT (target)
Isogenic modification
17q21.31
NM_016834.4 : c.[ c.727 >T; c.[741+16C>T]+[741+16C>T]]
P301S+IVS10+16 C>T; Regarding homozygosity/heterozygosity-
Mutated
corticobasal degeneration
There are three disease associations for the edited subclone: PSP (progressive supranuclear palsy) /CBD (Corticobasal degeneration) / FTDP-17 (Frontotemporal dementia and parkinsonism linked to chromosome 17) - like symptoms
Genetic modifications
MAPT (target)
Isogenic modification
17q21.31
NM_016834.4 : c.[ c.727 >T; c.[741+16C>T]+[741+16C>T]]
P301S+IVS10+16 C>T; Regarding homozygosity/heterozygosity-
Mutated
FTDP-17 (Frontotemporal dementia and parkinsonism linked to chromosome 17) has no ontology id.
Genetic modifications
MAPT (target)
Isogenic modification
17q21.31
NM_016834.4 : c.[ c.727 >T; c.[741+16C>T]+[741+16C>T]]
P301S+IVS10+16 C>T; Regarding homozygosity/heterozygosity-
Mutated