iPSC0028 MAPT P301S+Ex10+16/Clone 7C6A4, SAMEA4451118

General

Cell Line

hPSCreg name SIGi001-A-11
Cite as:
SIGi001-A-11 (RRID:CVCL_LE50)
Alternative name(s)
iPSC0028 MAPT P301S+Ex10+16/Clone 7C6A4, SAMEA4451118
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
SIGi001-A-8
(iPSC0028 MAPT P301S+Ex10+16/Clone 7D11A7)
SIGi001-A-9
(iPSC0028 MAPT P301S+Ex10+16/Clone 7H6A1)
SIGi001-A-10
(iPSC0028 MAPT P301S+Ex10+16/Clone 7G4A8)
SIGi001-A-3
(iPSC0028 MAPT P301L C4)
SIGi001-A-4
(iPSC0028 MAPT P301L D4)
SIGi001-A-5
(iPSC0028 MAPT P301S 1C9C4)
SIGi001-A-6
(iPSC0028 MAPT P301S 1B9C9)
SIGi001-A-7
(iPSC0028 MAPT P301S 2G2B7)
SIGi001-A-12
(iPSC0028 - BiAllelic MAPT_Ex10+16T/Clone 1F5-D12, SAMEA104237570)
SIGi001-A-16
(SIGi001-A-9 Bi-Allelic MAPT HA-tag)
SIGi001-A-13
(iPSC0028 – MonoAllelic MAPT_Ex10+16T/Clone 1D01-11)
SIGi001-A
(IPSC0028 parental, SAMEA4447455)
SIGi001-A-1
(iPSC0028 SLC17A7/GFP E3)
SIGi001-A-2
(iPSC0028 SLC17A7/GFP C3)
SIGi001-A-21
(SIGi001-AAVS1-FRT)
SIGi001-A-22
(SIGi001-ICAM1-GFP)
SIGi001-A-23
(SIGi001-HC3x)
SIGi001-A-24
(SIGi001-HC3x-ICAM1-GFP)
SIGi001-A-14
(SIGi001-A Bi-Allelic MAPT HA-tag)
SIGi001-A-15
(SIGi001-A Dox inducible NGN2)
SIGi001-A-17
(SIGi001-A-9 Dox inducible NGN2)
SIGi001-A-18
(SIGi001-A-12 Bi-Allelic MAPT HA-tag #U28-12-P29-5)
SIGi001-A-19
(SIGi001-A-12 Dox inducible NGN2)
KUi013-A-1
(SK GC)
Last update 13th May 2024
Notes The parental iPSC line SIGi001-A can be sourced via Sigma as 'iPSC0028'.
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Provider

Generator Sigma-Aldrich (SIG)
Distributors

External Databases

BioSamples SAMEA4451118
Cellosaurus CVCL_LE50
Wikidata Q54953432

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history no
Is the medical history available upon request? no
Is clinical information available? no

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA4447434

Ethics

Also have a look at the ethics information for the parental line SIGi001-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line SIGi001-A.

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
CXCR4
Yes
GATA6
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
VIM
Yes
MIXL1
Yes
NCAM1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
NeuroD1
Yes
PAX6
Yes
HES5
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 37

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX[17]/47,XX,+12[2]
Passage number: P37
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755
This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee https://ega-archive.org/dacs/EGAC00001000768

Genetic Modification

Disease/phenotype related modifications
There are three disease associations for the edited subclone: PSP (progressive supranuclear palsy) /CBD (Corticobasal degeneration) / FTDP-17 (Frontotemporal dementia and parkinsonism linked to chromosome 17) - like symptoms
Synonyms
  • PSP syndrome
Genetic modifications
MAPT (target)
Isogenic modification
17q21.31
NM_016834.4 : c.[ c.727 >T; c.[741+16C>T]
Heterozygous
P301S+IVS10+16 C>T; both variants are heterozygous
Mutated
There are three disease associations for the edited subclone: PSP (progressive supranuclear palsy) /CBD (Corticobasal degeneration) / FTDP-17 (Frontotemporal dementia and parkinsonism linked to chromosome 17) - like symptoms
Synonyms
  • cortical basal ganglionic degeneration
  • corticobasal degeneration
  • CBGD
  • cortical-basal ganglionic degeneration
  • cortico-basal ganglionic Degeneration (CBGD)
  • corticobasal syndrome
  • corticodentatonigral degeneration with neuronal achromasia
show more synonyms
Genetic modifications
MAPT (target)
Isogenic modification
17q21.31
NM_016834.4 : c.[ c.727 >T; c.[741+16C>T]
Heterozygous
P301S+IVS10+16 C>T; both variants are heterozygous
Mutated
Genetic modifications
MAPT (target)
Isogenic modification
17q21.31
NM_016834.4 : c.[ c.727 >T; c.[741+16C>T]
Heterozygous
P301S+IVS10+16 C>T; both variants are heterozygous
Mutated