iPSC0028 MAPT P301S+Ex10+16/Clone 7C6A4, SAMEA4451118
SIGi001-A-11
General#
Cell Line |
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| hPSCreg Name | SIGi001-A-11 |
| Alternative name(s) |
iPSC0028 MAPT P301S+Ex10+16/Clone 7C6A4, SAMEA4451118
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 23rd October 2017 |
| User feedback | |
Provider |
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| Generator | Sigma-Aldrich (SIG) |
| Distributors | |
External Databases |
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| BioSamples | SAMEA4451118 |
| Cellosaurus | CVCL_LE50 |
| ECACC | 66540359 |
| EBiSC | SIGi001-A-11 |
General Information |
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| Is the cell line available in principle? | Available with restrictions: Available for clinical and commercial research |
| Subclone of | |
Donor Information#
General Donor Information |
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| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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External Databases (Donor) |
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| BioSamples | SAMEA4447434 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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The source cell information can be found in the parental cell line
SIGi001-A.
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Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Retrovirus) |
| Genes | |
| Is the used vector excisable? |
No |
| Absence of reprogramming vector(s)? |
Unkown |
| Reprogramming vectors silenced? |
Yes |
| Methods used |
RT-PCR
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Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 21 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| SSEA-1 |
No |
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Differentiation Potency
In vitro spontaneous differentiation
In vitro spontaneous differentiation
In vitro spontaneous differentiation
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46,XX[17]/47,XX,+12[2]
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification#
| Disease/phenotype related modifications |
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