BIONi010-C, K3P53

General#

Cell Line

hPSCreg Name BIONi010-C
Alternative name(s)
BIONi010-C, K3P53
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th August 2018
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Provider

Generator Bioneer (BION)
Contact:
Bioneer (BSC)
Owner Bioneer (BSC)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA3158050
Cellosaurus CVCL_1E68
ECACC 66540023
EBiSC BIONi010-C

General Information

Notes No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23
Publications
Projects
Is the cell line available in principle? Available, without restrictions
Subclones

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell line name CC-2511
Derived from same source line (potentially other lot and donor, see below):
Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Source cell type (free text) NHDF-Ad-Der Fibroblasts
Source cell line lot number
0000208364
Derived from same source line lot:
Age of donor (at collection) 15-19
Source cell line vendor LONZA

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Dispase
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Unkown
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
ZFP42 (REX-1)
Unkown
DNMT3B
Yes
GDF3
Unkown
nodal
Unkown
podxl
Unkown
xist
Unkown
LEFTY1
Unkown
foxd3
Unkown
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
SMA
Yes
Neuron
Ont Id: CL_0000540
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
Tuj1
Yes
Nestin
Yes
VIMENTIN
Yes
vGLUT1
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array
No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23