BIONi010-C-41

BIONi010-C + iNGN2 Two-plasmid system/CRISPR-2

General

Cell Line

hPSCreg Name
BIONi010-C-41
Alternative name(s)
BIONi010-C + iNGN2 Two-plasmid system/CRISPR-2
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd December 2022
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Provider

Generator Bioneer (BION)
Owner Bioneer (BION)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA9349208

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Black or African-American

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

Ethics

Also have a look at the ethics information for the parental line BIONi010-C .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line BIONi010-C.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ Plus

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-1
No
TRA 1-60
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
CXCR4
Yes
Gata6
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
VIM
Yes
DCN
No
MIXL1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
NEUROD1
No
PAX6
Yes
HES5
Yes

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
Transgene expression
AAVS1
Doxycycline inducible iNGN2 system (iNGN2 Two-template system/CRISPR-2)
CRISPR-associated (CRISPR/Cas) System