BIONi010-C + iNGN2 Two-plasmid system/CRISPR-2
BIONi010-C-41
General
Cell Line |
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| hPSCreg Name | BIONi010-C-41 |
| Alternative name(s) |
BIONi010-C + iNGN2 Two-plasmid system/CRISPR-2
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 22nd December 2022 |
| User feedback | |
Provider |
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| Generator | Bioneer (BION) |
| Owner | Bioneer (BION) |
| Distributors | |
| Derivation country | Denmark |
External Databases |
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| BioSamples | SAMEA9349208 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Black or African-American |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA3105780 |
Ethics
Also have a look at the ethics information for the parental line
BIONi010-C
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
BIONi010-C.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Methods used |
PCR
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| SSEA-1 |
No |
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| TRA 1-60 |
Yes |
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| SSEA-4 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
In vitro directed differentiation
Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
Genetic Modification
| Genetic modifications not related to a disease |
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