BIONi010-C-71

BIONi010-C with an APOE 3/3 genotype with an additional, homozygous christchurch mutation

General

Cell Line

hPSCreg name BIONi010-C-71
Cite as:
BIONi010-C-71 (RRID:CVCL_D6RT)
Alternative name(s)
BIONi010-C with an APOE 3/3 genotype with an additional, homozygous christchurch mutation
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
BIONi010-C
(BIONi010-C, K3P53)
BIONi010-C-3
(BIONi010-C ApoE KO #KO30 P30)
BIONi010-C-25
(BIONi010-C heterozygous TREM2 KO)
BIONi010-C-17
(BIONi010-C TREM2 KO)
BIONi010-C-2
(BIONi010-C ApoE E3/E3 #H8 P32)
BIONi010-C-70
(BIONi010-C with an APOE 2/2 genotype with an additional, homozygous christchurch mutation)
BIONi010-C-4
(BIONi010-C ApoE E4/E4 #B44 P27)
BIONi010-C-5
(BIONi010-C CD33 E2del #N14 P26)
BIONi010-C-6
(BIONi010-C ApoE E2/E2)
BIONi010-C-7
(BIONi010-C Trem2 R47H)
BIONi010-C-8
(BIONi010-C Trem2 T66M, #Y5-80)
BIONi010-C-9
(BIONi010-C CD33 KO)
BIONi010-C-18
(BIONi010-C TBK1 KO)
BIONi010-C-13
(BIONi010-C + NGN2 #I7-26)
BIONi010-C-15
(BIONi010-C +dox inducible NGN2-GFP)
BIONi010-C-51
(BIONi010-C TNNI3-mCherry reporter)
BIONi010-C-19
(BIONi010-C IKBKE KO)
BIONi010-C-10
(HNF1AP291fsinsC +/- 54-5)
BIONi010-C-11
(HNF1AP291fsinsC -/- 66-1)
BIONi010-C-12
(HNF4ApR309C -/- 2-4)
BIONi010-C-52
(BIONi010-C with an APOE 2/2 genotype (with two functional alleles in contrast to BIONi010-C-6))
BIONi010-C-53
(BIONi010-C with an APOE 3/3 genotype (with two functional alleles in contrast to BIONi010-C-2))
BIONi010-C-55
(BIONi010-C TNNI3-mCherry/TNNI1-EGFP dual reporter cl. 74)
BIONi010-C-24
(BIONi010-C Dox a-syn)
BIONi010-C-43
(BIONi010-C + aSNCA-wt AAVS1)
BIONi010-C-44
(BIONi010-C + aSNCA-A53T AAVS1)
BIONi010-C-54
(BIONi010-C with an APOE 4/4 genotype (with two functional alleles in contrast to BIONi010-C-4))
BIONi010-C-48
(BIONi010-C hMDR1)
BIONi010-C-45
(BIONi010-C iCRE AAVS1 GBA1 LoxP EX5-6)
BIONi010-C-41
(BIONi010-C + iNGN2 Two-plasmid system/CRISPR-2)
BIONi010-C-42
(BIONi010-C + iCRE AAVS1)
BIONi010-A
(K1P53)
BIONi010-B
(K2P53, BIONi010-B)
UKBi011-A-3
(ApoE 3/3)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-4
(ApoE 3/4)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-1
(iLB-AD + ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-2
(ApoE 2/2)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A-1
(ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A-3
(ApoE 3/3)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
STBCi006-A-4
(ApoE 3/4)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UMi042-A-1
(408737 CB9)
Donor diseases:
Alzheimer's Disease
UCSFi001-A-74
(FUS-P525L HET 2D1)
UCSFi001-A-75
(FUS-P525L HOM 2D2)
WTSIi018-B-18
(Kolf 2.1 J (TARDBP M337V WT/SNV))
UQi001-A-1
(C11-TDP43-A382T)
WTSIi018-B-21
(Kolf 2.1 J (TARDBP M337V SNV/SNV))
WTSIi018-B-22
(Kolf 2.1 J (TARDBP Q331K WT/SNV))
WTSIi018-B-23
(Kolf 2.1 J (TARDBP Q331K SNV/SNV))
WTSIi018-B-24
(Kolf 2.1 J (FUS R495X WT/SNV))
Last update 30th January 2024
Notes The line has an APOE 3/3 genotype with an additional, homozygous christchurch mutation
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Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BSC)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA115162593
Cellosaurus CVCL_D6RT

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Black or African-American

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XY
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

Ethics

Also have a look at the ethics information for the parental line BIONi010-C .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line BIONi010-C.
Passage number reprogrammed 32

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium mTeSR™ Plus

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
TRA 1-81
Yes
SSEA-1
No
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 32

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
The line has an APOE 3/3 genotype with an additional, homozygous christchurch mutation
Synonyms
  • Alzheimer's Disease
  • Alzheimer disease
  • Alzheimer Disease
  • Alzheimer dementia
  • Alzheimer's Dementia
  • Alzheimer's disease
  • Alzheimer's disease, unspecified
show more synonyms
Genetic modifications
Variant
The line has an APOE 3/3 genotype with an additional, homozygous christchurch mutation