BIONi010-C-10

HNF1AP291fsinsC +/- 54-5

General#

Cell Line

hPSCreg Name BIONi010-C-10
Alternative name(s)
HNF1AP291fsinsC +/- 54-5
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th August 2018
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Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA104619380
EBiSC BIONi010-C-10
ECACC 66541074
Cellosaurus CVCL_RM84

General Information

Notes No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23
Is the cell line available in principle? Available, without restrictions
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

The source cell information can be found in the parental cell line BIONi010-C.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Method documentation
HNF1A 54-5 flow.tif
Flow cytometry of undifferentiated cells

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
MODY
Synonyms
  • Maturity-onset diabetes of the young
Genetic modifications
HNF1A (target)
Isogenic modification
12q24.31
NM_000545.6:c.(c.872dup)
NP_000536.5:p.(P291fsinsC)
Heterozygous
An additional cytocine has been introduced by CRISPR-Cas9 into a poly-C tract in Exon 4 of HNF1A resulting in a out of frame mutation. It is likely that the other allele has also been targeted, since western blotting did not show any HNF1A protein in Pancreatic progenitor cells compared to the control line BIONi010-C.
Mutated
HNF1A 54-5 BIONi010-C.tif
Sequencing of HNF1A 54-5 and BIONi010-C showing a heterozygous mutation in one of the alleles of HNF1A