BIONi010-C-52

BIONi010-C with an APOE 2/2 genotype (with two functional alleles in contrast to BIONi010-C-6)

General

Cell Line

hPSCreg Name
BIONi010-C-52
Alternative name(s)
BIONi010-C with an APOE 2/2 genotype (with two functional alleles in contrast to BIONi010-C-6)
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 1st February 2023
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Provider

Generator Bioneer (BION)
Contact:
EBiSC
Owner Bioneer (BION)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA8093245
CLO CLO_0104076

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Black or African-American

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

Ethics

Also have a look at the ethics information for the parental line BIONi010-C .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

The source cell information can be found in the parental cell line BIONi010-C.
Passage number reprogrammed 36

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Non coated
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line BIONi010-C .

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
Isogenic modification
Homozygous
APOE2/2
Mutated