BIONi010-C-18

BIONi010-C TBK1 KO

General#

Cell Line

hPSCreg Name BIONi010-C-18
Alternative name(s)
BIONi010-C TBK1 KO
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th August 2018
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Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA104491010
EBiSC BIONi010-C-18
ECACC 66540637
Cellosaurus CVCL_RM89

General Information

Notes No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23
Is the cell line available in principle? Available, without restrictions
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

The source cell information can be found in the parental cell line BIONi010-C.

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Morphology pictures
TREM2 KO cl. 24-54 D1 thaw after banking.tif
Morphology 1 day after thawing
Method documentation
qPCR BIONi010-C-18.tif
qPCR analysis of undifferentiated cells
06-Sep-2017-Layout.tiff
Flow cytometry analysis of undifferentiated cells
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Morphology
BIONi10-C18 endo d5 10x.tiff
Morphology of in vitro diff to endoderm
Protocol or reference
BIONi10-C18 endo d5.tiff
Flow Cytometry of in vitro differentiation to endoderm
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Morphology
BIONi10-C18 meso d5 10x.tiff
Morphology of in vitro diff to mesoderm
Protocol or reference
BIONi10-C18 meso d5.tiff
Flow Cytometry of in vitro differentiation to mesoderm
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Morphology
BIONi10-C18 ecto d5 10x.tiff
Morphology of in vitro diff to ectoderm
Protocol or reference
BIONi10-C18 ecto d5.tiff
Flow Cytometry of in vitro differentiation to ectoderm

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
inflammatory diseases and cancer
Genetic modifications
TBK1 (target)
Gene knock-out
KO of exon 2 of TBK1 (NM_013254)
CRISPR-associated (CRISPR/Cas) System
TBK1 KO BIONI010-C-18.pdf
Sequence and confirmation of single cell clone of BIONi010-C-18