BIONi010-C TBK1 KO
BIONi010-C-18
General
Cell Line |
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hPSCreg Name | BIONi010-C-18 |
Alternative name(s) |
BIONi010-C TBK1 KO
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 7th March 2023 |
Notes | No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23 |
User feedback | |
Provider |
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Generator |
Bioneer (BION)
Contact:
Bioneer (BION) |
Owner | Bioneer (BION) |
Distributors | |
Derivation country | Denmark |
External Databases |
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BioSamples | SAMEA104491010 |
EBiSC | BIONi010-C-18 |
ECACC | 66540637 |
Cellosaurus | CVCL_RM89 |
CLO | CLO_0102730 |
Wikidata | Q54796763 |
General Information |
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Projects | |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Subclone of |
Donor Information
General Donor Information |
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Sex | male |
Ethnicity | Black or African-American |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
|
BioSamples | SAMEA3105780 |
Ethics
Also have a look at the ethics information for the parental line
BIONi010-C
.
Is there an MTA available for the cell line? | Yes |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
BIONi010-C.
|
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Reprogramming method |
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Vector type | Non-integrating |
Vector | Episomal |
Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
Morphology pictures
TREM2 KO cl. 24-54 D1 thaw after banking.tif
Morphology 1 day after thawing
Method documentation
qPCR BIONi010-C-18.tif
qPCR analysis of undifferentiated cells
06-Sep-2017-Layout.tiff
Flow cytometry analysis of undifferentiated cells
Differentiation Potency
In vitro directed differentiation
Morphology
BIONi10-C18 endo d5 10x.tiff
Morphology of in vitro diff to endoderm
Protocol or reference
BIONi10-C18 endo d5.tiff
Flow Cytometry of in vitro differentiation to endoderm
In vitro directed differentiation
Morphology
BIONi10-C18 meso d5 10x.tiff
Morphology of in vitro diff to mesoderm
Protocol or reference
BIONi10-C18 meso d5.tiff
Flow Cytometry of in vitro differentiation to mesoderm
In vitro directed differentiation
Morphology
BIONi10-C18 ecto d5 10x.tiff
Morphology of in vitro diff to ectoderm
Protocol or reference
BIONi10-C18 ecto d5.tiff
Flow Cytometry of in vitro differentiation to ectoderm
Microbiology / Virus Screening |
|
Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
Genetic Modification
Disease/phenotype related modifications |
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